摘要
目的探讨正常状态下不同胎龄大鼠神经干细胞(neuralstemeells,NSCs)在大脑中的分布。方法将孕鼠随机分为胎龄11天组、15天组和19天组,用免疫组织化学方法检测其大脑神经干细胞中的巢蛋白(Nestin),经图像分析比较其差异。结果胎龄11天组Nestin阳性细胞分布于整个神经管上皮层,随着胎龄的增加,Nestin阳性细胞主要在室管膜上皮层,细胞数逐渐增加,并向周围迁移;结论大鼠在正常胚胎发育过程中,随着胎龄的增加,其脑室室管膜及室管膜下区神经干细胞逐渐增多。
Objective To study the contribution of neural stem cells(NSCs)in rats from diffent embryonic days. Methods 12 female Wistar rats were divided randomly into three groups: embryonic days 11 group(E11) 、 (E15) and E19. The expression of Nestin in brain of embryos was studied with immunohistochmistry and the quantity of postive cells were analyzed by image processing system. Result In E11,the Nestin-postive cells were contributed throughout neural tube. With the increasing of the embryonic days, they aggregated mainly in ependymal and subependymal zone, and the number of Nestin-postive cells increased gradually. Conclusion With the increasing of the embryonic days, the NSCs in brain increase gradually in the proceeding of the normal development of embryo of rat.
出处
《四川解剖学杂志》
2005年第2期1-3,共3页
Sichuan Journal of Anatomy
关键词
胎鼠
神经干细胞
Embryonic rat
Neural stem cells
