恒河猴慢性青光眼模型的建立及相关生物学特性

Establishment of Chronic Glaucoma Model in Rhesus Monkeys and Evaluation of Their Related Biological Characteristics

目的建立两种激光光凝的恒河猴慢性高眼压性青光眼模型,评价模型眼的相关生物学特性。方法成年恒河猴15只,分别采用半导体倍频532激光和氩激光,在房角镜下对功能小梁网区行360°光凝。对其中7只恒河猴分别采用A超、视网膜断层成像仪和视网膜血流仪进行模型眼和另侧对照眼的眼球及视盘形态、血流参数的检测。结果两种光凝模式相比,眼压升高后第4周,倍频532激光组平均眼压为48.4±10.3mmHg,氩激光组平均眼压为44.2±7.0mmHg,倍频532激光组与氩激光组的三次光凝成功率的差异无显著性意义。除视盘面积外,恒河猴模型眼的视杯形态指数、杯盘面积比、盘沿面积、视网膜神经纤维层的平均厚度,与对照眼相比差异有极显著性意义。眼轴和前房深度与对照眼相比差异有显著性意义。筛板血流量、血流速度和红细胞移动速率与对照眼相比差异无显著性意义。结论两种激光光凝恒河猴小梁网均可用以建立慢性高眼压性青光眼模型,模型眼出现青光眼眼底特征性的形态学改变。

Objective To establish a chronic hypertensive glaucoma model by two types of laser photocoagulation in rhesus monkeys and to evaluate the related biological characteristics in the model eyes. Methods\ Laser photocoagulation was applied to 15 adult rhesus monkeys by semiconductor frequency doubled 532 laser and argon laser. The laser spot was aimed at the entire 360° functional trabecular meshwork using goniolens. A sonoscan,Heidelberg retinal tomograph and retinal flowmeter were used to examine the topographic and blood flow parameters of the globe and optic disc in seven model eyes and control eyes. Results At the fourth week after initial pressure elevation, the average intraocular pressure was 48 4±10 3 mmHg in eyes of frequency doubled 532?nm laser group and 44 2±7 0 mmHg in the argon laser group. There was no significant difference between the successful rates of three times photocoagulation obtained in the two groups. Compared with the control eyes, there were very significant differences of cup shape measure, cup area, cup/disc area ratio, rim area and mean retinal nerve fiber layer thickness in the model eyes, except for the disc area. There were also significant differences of axial length and anterior chamber depth between them. There was no significant difference of the volume, flow and velocity between the model eyes and control eyes. Conclusions\ Both the two types of laser photocoagulation successfully induced chronic hypertensive glaucoma model in rhesus monkey eyes. Compared with the control eyes, the model eyes present characteristic glaucomatous morphological changes.