猪脑肽溶液对衰老小鼠超氧化物歧化酶以及丙二醛的影响(英文)

Effects of pig brain peptide solution on erythrocuprein and malonaldehyde in senile mice

背景:随着年龄的增加,脑内超氧化物歧化酶活性降低、丙二醛含量增加而造成的损伤作用越来越受到重视;猪脑肽溶液能否通过血脑屏障进入脑组织对脑神经起保护作用,目前只是临床病例报道,对其实验研究国、内外报道较少。目的:探讨猪脑肽溶液对D-半乳糖拟衰老小鼠脑以及肝脏组织中丙二醛活性和超氧化物歧化酶含量的影响。设计:以实验动物为研究对象的随机对照实验观察。单位:解放军总医院老年医学研究所。材料:实验于2001-02/2001-04在解放军总医院老年医学研究所完成(军队重点实验室)。选择NH小鼠82只,雌雄各半,解放军总医院医学实验动物中心(清洁级)提供,体质量18～22g,随机分为对照组、猪脑肽溶液(施普善)大、中、小剂量组、阿尼西坦组(阳性对照组)和D-半乳糖组。方法:各组分别给药,连续给6周,采用D-半乳糖诱导的脑老化小鼠动物模型。各组分别用化学发光法测定超氧化物歧化酶活性,用硫代巴比妥酸比色法测定MDA含量。主要观察指标:各组小鼠的SOD活性和丙二醛含量。结果:猪脑肽溶液大、中剂量均能显著提高脑以及肝脏组织中超氧化物歧化酶活性[分别为(30.23±5.23),(29.65±4.88);(19.84±5.79),(16.75±5.32)μkat/g],降低丙二醛含量[分别为(630.00±59.40),(626.74±49.68);(934.90±207.50),(916.06±235.31)nmol/g],与D-半乳糖组比较[分别为(22.89±4.54),(12.26±6.31)μkat/g;(749.69±92.80),(1212.89±142.54)nmol/g],差异均有显著性意义(P<0.05或P<0.01)。结论:猪脑肽溶液具有明显的抗氧化能力,对衰老小鼠脑神经细胞及肝细胞有一定的保护作用,为评估其对老年机体脑及肝细胞的抗自由基氧化损伤作用提供理论支持。

BACKGROUND:More attention has been paid upon the injury effect resulted from the decrease of superoxide dismutase(SOD)activity and increase of malonaldehyde(MDA)content in brain.Whether pig brain peptide solution can pass bloodbrain barrier and penetrate into brain tissue and protect brain nerve is still found in limited cases reports.Relevant reports are still rare both in China and abroad.OBJECTIVE:To explore the effect of pig brain peptide solution on SOD activity and MDA content in brain and liver of senile model mice made by Dgalactose.DESIGN:Experimental animalsbased randomized and controlled experimental observation.SETTING:Institute of Senile Medical of General Hospital of Chinese PLA.MATERIALS:The experimant was made in the Institute of Senile Medical(Chinese PLA key laboratory) of General Hospital of Chinese PLA from February to April 2001.Totally 82 NH mice,weighted from 18 to 22 g,half male and half female, were provided by the Medical Experimental Animal Center of General Hospital of Chinese PLA.These mice were randomly divided into the control group,hige dose group, middle dose group,low dose group,aniracetam group(positive control group),and Dgalactose group.METHODS:Each group was given drugs seperately for 6 weeks. The senile mice model was induced by Dgalactose.SOD activity of each group was checked with chemiluminescence method and MDA content examined with thiobarbituric acid colourimetry method.MAIN OUTCOME MEASURES:SOD activity and MDA content of mice in each group.RESULTS:Pig brain peptide solution with high and middle doses significantly increased SOD activity[(30.23±5.23),(29.65±4.88);(19.84±5.79),(16.75±5.32)μkat/g respectively], and decreased MDA content[(630.00±59.40),(626.74±49.68);(934.90±207.50),(916.06±235.31)nmol/g respectively] in brain and liver tissues. Compared with Dgalacose group[(22.89±4.54),(12.26±6.31)μkat/g;(749.69±92.80),(1 212.89±142.54)nmol/g].Significant differences existed(P< 0.05 or P< 0.01).CONCLUSION:Pig brain peptide solution has obvious antioxidative effect, and has certain protective effects on brain nerve cells and liver cells of senile mice.