海洋鱼蛋白可控酶解动力学模型的研究

Study on kinetic model of controllable-hydrolysis of sea-fish protein

应用数学推导结合实验研究的方法,以沙丁鱼蛋白为底物,研究胰蛋白酶可控酶解过程的动力学模型及其相关参数,结果如下:DH=9.804ln[1+(1·701E0/S0-0·00367)]和R=(16·678E0-0·036S0)exp[-0·102(DH)],动力学常数k2=16.678/min和kd=1·7015/min,最低临界初始蛋白酶浓度E0=c0S0,最大临界底物初始浓度S0=E0/c0,c0=2·159×10-3。与实验结果相比较,证实动力学模型与实验结果非常吻合,说明它具有很好的实际应用价值。

The kinetic model and relative parameter of controllable-enzymatic hydrolysis were obtained by use of mathematic deduction and experimental analysis.The result is as follows:kinetic model formula is DH=9.804 ln [1+(1.701E_0/S_0-0.00367)t]and R=(16.678E_0-0.036S_0)exp[-0.102(DH)];kinetic parameter is k_2=16.678min^(-1)k_d=1.7015 min^(-1);the lowest,critical initial concentration of protease and the highest,critical initial concentration of substrate S_0=E_0/c_0,and c_0=2.159×10^(-3) respectively.Comparied with experiment result,the kinetic model fits well.It shows that the kinetic model has significant application value.