妊娠高血压综合征与MTHFR A1298C基因及血浆同型半胱氨酸水平的关系

The relationship between polymorphism of methylenetetrahydrofolate reductase(MTHFR A1298C) gene and pregnancy induced hypertension syndrome

目的探讨妊娠高血压综合征与N5,N10鄄亚甲基四氢叶酸还原酶(MTHFRA1298C)基因及血浆同型半胱氨酸(Hcy)水平的关系。方法运用多聚酶链反应鄄限制性内切酶片段长度多态性技术(PCR鄄RFLP)检测54例妊高征患者,100例正常妊娠妇女(MTHFRA1298C基因多态性,采用荧光偏振免疫法(FPIA)测定血浆总Hcy水平。结果MTHFRA1298C三种基因型频率与对照组相比差异无显著性,与患者不同临床特征无明显关联。妊高征组总Hcy水平明显高于正常妊娠组(P<0.05)。结论MTHFRA1298C基因突变与妊高征发生无关;高同型半胱氨酸血症是妊高征发病的一个新的危险因素。

Objective To investigate the correlation between the polymorp hism of methylenetetrahydrofolate reductase(MTHFR A1298C) and the incidence of p regnancy induced hypertension syndrome(PIH). Methods The polymorphism of MTHFR A1298C and the level of plasma homocysteine in pregnancy women of normal and PIH were analyzed by means of PCR and RFLP. Results There were no siganificant dif ferences in the frequenceies of methylenetetrahydrofolate reductase mutations be tween the patients and contral groups. The level of plasma homocysteine in pregn ancy women of PIH was much higher than that in pregnancy women of normal group. Conclusion The point mutation of A1298C of MTHFR may have no relationship with PIH., Hcy may not be independent risk factors for PIH.