23SrDNA基因芯片对临床常见致病菌的检测

Detection of Clinical Infectious Bacteria by 23S Ribosomal DNA Microchips

目的建立含有10种细菌探针的检测用基因芯片模型。方法使用合成的23SrDNA寡核苷酸探针,制备基因芯片。细菌核糖体DNA经过23SrDNA通用引物扩增后与芯片上的探针杂交,用荧光扫描仪检测信号。结果基因芯片检测临床致病菌具有较高的特异性和灵敏性。结论寡核苷酸探针基因芯片检测系统具有一定的种属鉴别能力,比常规培养法快速、准确。

Objective To develop a gene chip model containing 10 probes for infectious bacteria.Methods Ten synthesized oligonucleotide 23S rDNA probes were employed to make gene chips by spoter. The DNA of bacteria was amplified by 23S rDNA universal primers and the PCR product was then applied to the gene chips for hybridization. A fluorescent scanner was used to observe and record the hybridization signals.Results The sensitivity and specificity was high in detecting bacterial 23S rDNA by gene chips method.Conclusion The oligonucleotide microarray could determine most of the test strains at species level. It is more rapid and accurate than routine culture method.