摘要
目的探讨灌胃给予肿瘤坏死因子α(TNFα)多克隆抗体对溃疡性结肠炎的作用。方法以50mg/kg三硝基苯磺酸(TNBS)经肛门注入大鼠肠腔,复制大鼠溃疡性结肠炎模型;用兔抗人TNFα多克隆抗体血清10mg/kg给大鼠灌胃,观察一氧化氮(NO)含量、髓过氧化物酶(MPO)活性及TNFα在大鼠溃疡性结肠炎组织中的表达。结果抗TNFα多克隆抗体治疗可明显减轻溃疡性结肠炎的病理改变,抑制局部结肠组织NO的产生、MPO活性以及TNFα表达。结论抗TNFα血清通过中和TNFα,可抑制溃疡性结肠炎结肠组织表达TNFα及炎性介质的释放,从而减轻溃疡性结肠炎的病理损伤。
Objective To investigate the effect of polyclonal antiboidy against TNF on ulcer colonitis by stomach perfusion.Methods Experimental colonitis model was obtained by challenging the rat with 50 mg/kg TNBS with colon perfusion through anus. NO content, MPO activity and TNF-alpha expression in the ulcer colonitis tissue were determined.Results Polyclonal antibody against TNF-alpha reduced the pathological alternations of ulcer colonitis tissue, inhibited NO production, MPO activity and TNF-alpha expression as well.Conclusion Anti-TNF polyclonal antibody can inhibit the TNF-alpha expression and other inflammatory mediators by neutralizing TNF-alpha, which reduces pathological changes in local colonitis tissue and plays an important role in therapeutic effect.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2005年第3期281-283,共3页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
国家高技术研究发展计划(863计划)资助项目(No.2004AA215160)
关键词
抗体
肿瘤坏死因子
溃疡性结肠炎
肿瘤坏死因子-Α
antibody,tunor necrosis factor-alpha
ulcer colonitis Crohn disease
tunor necrosis factor-alpha
