环状芽孢杆菌果胶酶及木聚糖酶活性测定条件优化

Optimum Method for Determination of Pectinase and Xylanase in Activity Bacillus circulans

为探明环状芽孢杆菌果胶酶及木聚糖酶的催化特性,更好发挥它们的催化性能,从pH值、温度、底物浓度、反应时间、离子浓度几个方面研究了环状芽孢杆菌A6中果胶酶与木聚糖酶活性测定的最适方法.结果表明,果胶酶活性测定的最适条件是:0.5%的果胶溶液用pH10.5的0.2mol/L甘氨酸-氢氧化钠缓冲液配制,测定温度45℃,酶反应时间5min;木聚糖酶活性测定的最适条件是:0.8%的木聚糖溶液用pH7.0的0.2mol/L磷酸缓冲液配制,测定温度55℃,酶反应时间10min.

To find out the catalysis character of pectinase and xylanse in bacillus circulans and exhibit their maxium catalysis rate,that the optimum condition for determination of pectinase and xylanase activity in Bacillus circulans was studied in aspect of pH,temperature,substance mass rate,reaction time,anion concentration.The results showed the optimum condition for determination pectinase activity:0.5% pectine resolution,pH 10.5,0.2 mol/L glycine-sodium hydrooxide buffer,45 ℃,five-minute reaction time;for of xylanase activity,its was:0.8% xylane resolution,pH 7.0,0.2 mol/L phoslate buffer,55 ℃,five-minute reaction time.