摘要
目的:研究17β-雌二醇对血管性痴呆(VaD)大鼠认知功能和脑组织神经生长因子(NGF)、脑源性神经营养因子(BDNF)和胶质细胞源性神经营养因子(GDNF)表达的影响。方法:将24只雄性Wistar大鼠随机分入假手术组、17β-雌二醇组和赋形剂组,每组8只。17β-雌二醇组腹腔注射17β-雌二醇(1mg/kg,2次/周),赋形剂组腹腔注射等量消毒花生油。采用结扎双侧颈总动脉法制备慢性前脑缺血即VaD动物模型,应用Y迷宫检测大鼠认知功能,应用免疫组化法检测脑组织中NGF、BNDF和GDNF的含量变化。结果:17β-雌二醇组60d后的认知功能明显好于赋形剂组(P<0.01),脑组织内NGF、BDNF和GDNF阳性神经元显著多于赋形剂组(P<0.01)。结论:腹腔注射17β-雌二醇可显著改善VaD大鼠的认知功能,增加VaD大鼠脑内的NGF、BDNF和GDNF的含量。
Objective: To investigate the effects of 17β-estradiol on cognitive function and the expressions of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) in vascular dementia (VaD) in rats. Methods: Twenty-four male Wister rats were randomly divided into the sham-operated group, the 17β-estradiol group and the vehicle group (8 rats each group). 17β-Estradiol (1 mg/kg) or vehicle was administered (1 mg/kg, twice a week) intraperitoneally after forebrain ischemia. The model of chronic forebrain ischemia (ie VaD animal model) was prepared by bilateral common carotid arteries ligation. The Y-maze test was used to evaluate the cognitive function in rats. The expressions of NGF, BDNFand GDNF were assessed by immunohistochemistry. Results: Cognitive function was much better in the 17β-estradiol group than that in the vehicle group at 60 days ( P <0.01). NGF-, BDNG- or GDNF-positive neurons in the brains of the 17β-estradiol group were significantly more than those in the vehicle group at 60 days ( P <0.01). Conclusion: The administration of 17β-estradiol intraperitoneally may significantly improve the cognitive function in VaD rats and increase their expressions of NGF, BDNF and GDNF in the brains.
出处
《国外医学（脑血管疾病分册）》
2005年第5期345-347,共3页
Foreign Medical Sciences Cerebrovascular Diseases
