卵巢癌患者接受抗CEA鼠单克隆抗体C_（50）放射免疫显像后血清中人抗鼠抗体的检测

Detection of Serum Anti-murine Antibody in Ovarian Cancer Patients after Radioimmunoimaging with Murine Anti-Carcinoembryonic Antigen Monoclonal Antibody C_(50)

我们用ＥＬＩＳＡ对２２例接受抗ＣＥＡ单抗Ｃ（５０）放射免疫显像的卵巢癌病人进行了血清人抗鼠抗体（ＨＡＭＡ）产生的动态检测。４５．５％（１０／２２）的患者产生ＨＡＭＡ，人抗鼠抗体在给药后２～３周即可出现，４～５周达高峰，并在体内持续至少９个月。所产生的ＨＡＭＡ在双向琼脂扩散中仅与抗人ＩｇＧ有沉淀线，且与标准ＩｇＧ的沉淀线相连，与抗人Ｉｇ轻链ｋ、λ单抗、葡萄球菌Ａ蛋白、羊抗人ＩｇＧ均起反应，证明该抗体属于ＩｇＧ。

Human anti-murine antibody (HAMA) was detected in 22 patients with ovarian cancer after radioimmunoimaging with murine anti-carcinoembryonic antigen monoclonal antibody C_(50) In 45. 5% of patients(10/22) given a dose of C_(50) (2mg) radiolabeled with 222 MBq of ￣(131)I ,HAMA developed within 2 to 3 weeks after injection with its peak at 4￣(th) to 5￣(th) week and sustained for at least 36 weeks. HAMA belongs to IgG class and can bind protein A ,anti-human IgG polyclonal antibody and anti-human light-chain monoclonal antibody.