溶脲脲原体感染动物模型的建立──在泌尿生殖系中的分布

Establishment of all animal model with ureaplasma urealyticum:distribution of ureaplasma urealycum in urogenital system

将１００只雄性ＳＤ大鼠分为３组。Ａ组４５只；Ｂ组２２只；Ｃ组３３只。Ａ组每只动物经膀胱注入血清８型溶脲脲原体（ｕｒｅａｐｌａｓｍａｕｒｅａｌｙｔｉｃｕｍ，ＵＵ）菌株（１９６０），浓度为１０５ＣＣＵ／ｍｌ。Ｂ组处理方法同Ａ组，在接种３个月后给予美满霉素（２０～１００ｍｇ／ｋｇ体重），连续１４天。Ｃ组为对照组，每只动物膀胱注入等量溶脲脲原体液体培养基。结果表明，在Ａ组动物的膀胱（８７．５％）、睾丸（６２．５％）、附睾（５５．０％）、精囊（６５．０％）和前列腺（５７．５％）中均检出溶脲脲原体。Ｂ组溶脲脲原体检出率显著低于Ａ组。Ｃ组上述器官溶脲脲原体培养均为阴性。

One hundred male SD rats were divided into three groups:45 in group A,22 in group B and 33 in group C。 Each animal in group A were infected with ureaplasma urealyticum serotype 8 (T960)by injecting broth containing organisms(105CCU / ml)into the urinary bladder following laparotomy. Animals in group B were treated similarly in group A. After 3 month8,animals in group B were given minocin(20～100 mg / kg)for l4 days.As a control group animals in group C were injected with broth only.The results showed that ureaplasma urealyticum were detected from urinary bladders (87.5%),testes (62.5%),epididymis(55%),seminal vesicles(65%),and prostates(57.5%).The positive infection rate of ureaplasma urealyticum in group B is significantly lower than that in group A,while the animals in group C were all negative.