促生长素抑制素抑制人胎盘绒毛促性腺激素分泌的机理探讨

The mechanism of somatostatin on huluan chorionic gonadotropin secretion induced by EGF and GnRH in cultured early placenta

应用组织培养技术，对两种不同浓度的促生长素抑制素（ＧＨＩＨ）抑制早期胎盘人绒毛促性腺激素（ｈＣＧ）分泌作用机制进行了初步探讨。结果表明：ＧＨＩＨ（５００或１０００ｎｇ／ｍｌ，６或２４ｈ）单独作用对绒毛ｈＣＧ分泌无影响（Ｐ＞０．１）；对表皮生长因子（ＥＧＦ），黄体生成素释放激素刺激的ｈＣＧ分泌的抑制率分别为４２％及５１％。腺苷酸环化酶激动剂ｆｏｒｓｋｏｌｉｎ（１０μｍｏｌ／Ｌ）和蛋白激酶Ｃ（ＰＫＣ）的激动剂ＰＭＡ（１００μｍｏｌ／Ｌ）可明显刺激绒毛分泌ｈＣＧ；ＧＨＩＨ（１０００ｎｇ／ｍｌ）对佛波醇醋和ｆｏｓｋｏｌｉｎ刺激的ｈＣＧ分泌有明显的抑制作用。提示第二信使途径ｃＡＭＰ及ＰＫＣ系统可能介导了ＧｎＲＨ及ＥＧＦ刺激的ｈＣＧ分泌。

This work showed that human chorionic gonadotropin (hCG)secretion induced by EGFand GnRH was inhibited by somatostatin(500ng / ml, 1 000ng / ml)in cultured early placenta with the maximal suppression of 42 % and 5 1 % of EGF and GnRH respectively.Somatostatin suppressed hCG secretion in response to the activators,such as protein kinase C(phorbal 12-myristate 1 3 acetate, PMA)and adenvlate cyclase(forskolin).The results indicated somatostatin may affect hCG secretion induced by EGF or GnRH by blocking adenylate cyclase and protein kinase C system.