酶-离子色谱法检测血清中的葡萄糖

Determination of Glucose in Serum by Enzyme-Ion Chromatography

利用葡萄糖氧化酶对β-D-葡萄糖催化氧化的高度专一性,催化性能高的优点,催化血清中β-D-葡萄糖被溶液中的溶解氧氧化为D-葡萄糖酸,同时结合离子色谱法快速、灵敏、准确的特点,检测生成的D-葡萄糖酸,并用标准工作曲线法来定量血清中的葡萄糖的含量,建立起酶-离子色谱法检测血清中葡萄糖的新方法.该法线性范围为1.00～90.00 mg/L.测得值与采用贝克曼CX3测得值相比,误差为-2%～2%.

β-D-Glucose in serum was oxidized by the oxygen dissolved in solution with the catalysis of glucose oxidase based on its high selectivity to glucose. We used the ion chromatography to determine the production, D-glucose acid and then the concentration of glucose in serum could be quantitatively determined through a standard working curve with a linear range of 1.00～90.00 mg/L.A new method has been developed to determine the glucose in serem.