原代培养细胞法测试水中类二噁英物质

Investigation of Dioxin-like Pollutants by Using Ethoxyresorufin Odeethylase with Primarily Cultured Hepatocyte from Grass Carp

采用固相萃取方法对北方某水厂水源水和各处理工艺出水进行富集,对各提取物采用草鱼原代培养肝细胞的7-乙氧基异吩唑酮-脱乙基酶(EROD)体外测试方法进行了类二口恶英物质的检验。结果表明,该水厂水源水和各处理工艺出水中,类二噁英物质浓度相当于0.20～0.50pg/L的2,3,7,8-TCDD(2,3,7,8-四氯代二苯并二噁英)当量,出水TCDD当量远远低于美国环保局饮用水二噁英类化合物含量标准(30pg/L)。研究表明,水样的固相萃取前处理方法结合原代培养细胞EROD分析,可以快速、有效地筛选和定量分析水样中未知的具有EROD效应的物质及其总体效应。

Water sample was collected from the source water and the treated water from different unit processes and then was enriched using solid phase extraction (SPE) method. The dioxin-like pollutants in the extracts was examined by using ethoxyresorufin O-deethylase (EROD) assay with primarily cultured hepatocyte from grass carp. The result shows that the concentration of dioxin-like pollutants in source water and treated water from each unit process is in the range of 0.20-0.50 pg/L of 2,3,7,8-TCDD (2,3,7,8-tetrachloro-p-dibenzodioxin) equivalent. TCDD in the finished water is much lower than the dioxin-like contaminant level in drinking water standard (30 pg/L) issued by USEPA. It is also verified that the SPE method for pretreatment of water sample in combination with EROD assay with primarily cultured hepatocyte can rapidly and effectively conduct screen and quantitative analysis for the unknown pollutants with EROD effect and the overall effect in the water sample.