摘要
探讨了蝴蝶兰的组织培养技术和方法。试验表明:用幼叶在散射光下培养诱导蝴蝶兰原球茎的效果最好,最适培养基为:MS+6-BA10mg/L+NAA1mg/L+CW20%+蔗糖20g/L+琼脂7g/L(pH值5.4);最适增殖培养基为:MS+6-BA2mg/L+NAA1mg/L+CW20%+蔗糖20g/L+琼脂7g/L(pH值5.4);最适生根长苗培养基为:1/2MS+NAA1mg/L+苹果酸30g/L+香蕉泥100g/L+活性炭1g/L+蔗糖15g/L+琼脂7g/L(pH值5.4)。
The experiment in the technology and system of tissue culture of Phalaenopsis was carried out. The results showed that the suitable induction medium for the PLBS(protocorm-like body of Phalaenopsis) was MS+ 6-BA 6 mg/L+ NAA 1 mg/L+ Ad 10 mg/L+ sugar 20 g/L+ coconut juice 20 % + agar 7.5 g/L (pH 5.4). The suitable propagation medium for the PLBS was MS + 6-BA 2 mg/L+ NAA 1 mg/L + Ad 10 mg/L+ sugar 20 g/L +coconut juice 20 %+agar 7.5 g/L (pH 5.4). The suitable regeneration medium was MS+NAA 1 mg/L + sugar 20 g/L+ active carbon 1 g/L+ banana mire 100 g/L+malic acid 30 mg/L+agar 7.5 g/L (pH 5.4).
出处
《安徽农业科学》
CAS
北大核心
2005年第6期1030-1031,共2页
Journal of Anhui Agricultural Sciences
基金
广东省科技攻关重点项目(C20304)
广州市科技攻关重点项目(200338)。
