L－天冬氨酸β－脱羧酶高活力菌株的培养

Culturing the Strain of High L-Aspartate β-decarboxylase Activity

为了获得较高的Ｌ－天冬氨酸β－脱羧酶活力，选用ＰｓｅｕｄｏｍｏｎａｓｄａｃｕｎｈａｅＣＰＵ９００１菌株进行培养，在含有１．５％Ｌ－谷氨酸盐，０．５％富马酸铵，１％富马酸钠，３．０％玉米浆，２％蛋白陈，０．０５％ＫＨ２ＰＯ４和０．０１％ＭｇＳＯ４·７Ｈ２Ｏ，ｐＨ７．０～７．５的培养液中３０℃发酵２８ｈ，Ｌ－谷氨酸盐有益于Ｌ－天冬氨酸β－脱羧醇的增加。

n order to obtain high L-Aspartate β-decarboxylase activity,cultural conditions of selected strains wereinvestigated. Pseudomonas dacunhae CPU9001 showed higther activity of L-Aspartate β-decarboxylaseunder aerobic conditions at 30℃ for 28 h in a medium(pH7.0-7.5)containing 1.5%L-glutamate,0.5%ammonium fumarate,1.0%sedium fumarate,3.0%corn steep liquor,2.0%pepton,0.05%KH2PO4,and 0.01%MgsO4.7H2O.L-glutamate was proved to be essential for increasing L-As-parate β-decarboxylase.