摘要
本文应用Ｔ％＝７％Ｃ％＝３．７％簿层聚丙烯酰胺凝胶等电聚焦（Ｔｈｉｎ－Ｌａｙｅｒｐｏｌｙａｃｒｙ－ｌａｍｉｄｅＧｅｌ－ＩｓｏｅｌｅｃｔｒｉｃＦｏｃｕｓｉｎｇ，ＴＬＰＡＧ－ＩＥＦ）技术电泳分离人红细胞溶血液和／或人红细胞ＳＯＤ抽提液，使用了一种自行设计的ＳＯＤ同工酶染色装置行特异性的ＳＯＤ同工酶染色。可将人红细胞ＳＯＤ分为１１条同工酶带。又根据酶带的ｐＩ、酶带理化性质、酶带特征和失活情况，分为三组同工酶。其中Ａ组ＳＯＤ同工酶为Ｃｕ、Ｚｎ＋ＳＯＤ，有三条（ｐＩ为４．５０、４．６５、４．７７），Ｂ组ＳＯＤ同工酶有三条（ｐＩ为４．８７、４．９５、５．１０），Ｃ组ＳＯＤ同工酶有五条（ＰＩ为５．４８、５．７５、５．８３、６．５２、８．４５），Ｂ、Ｃ两组ＳＯＤ同工酶既非Ｃｕ、Ｚｎ－ＳＯＤ，又非Ｍｎ－ＳＯＤ。其中Ｃ组ＳＯＤ同工酶特别容易失活。Ｂ、Ｃ两组ＳＯＤ同工酶的生理功用及临床意义尚不清楚。
emolyzed human erythrocyte solution or superoxide dismutase(SOD)extract
of human erythrocyteswas exdmined with electrophoresis using the thin-layer polyacrylamid
gel isoelectric focusing techniqueto separate the SOD isoenzymes when T%=7%and C%=3.7%,A
specific staining method devisedby the a uthors was used to stain the isoenzymes of SOD,It
was fou nd that the SOD isoenzvmes couldbe distinguished into 11 bands categorized into 3
groups according to the pl,physiochemical properties,characteristics,and the conditions of
inactivation of the isoenzyme bands. Group A consisted of Cu-andZn-SOD in 3 bands with the pl
of 4. 50,4.65 and 4. 77.Group B consisted of 3 bands with the pl of4.87,4. 95 and 5. 10.Group C
consisted of 5 bands with the pl of 5. 48,5. 75,5.83,6. 52 and 8.45.The SOD isoen zymes of
Grou ps Band C were neither Cu-and Zn-SOD non Mn-SOD and those ofGroup C were easily
inactivated. The physiological functions and clinical significance of the SOD isoen-zyemes of
Groupe B and C were not clear yet.
出处
《药物生物技术》
CAS
CSCD
1994年第2期26-32,共7页
Pharmaceutical Biotechnology
