原发性肝癌患者血清HBV一DNA及前C／C区变异的检测

DETECTI0N 0F HEPATITIS B VIRUS DNA PRECORE/CORE VARIATI0NS IN PATIENTS WITHHEPATOCELLULAR CARCINOMA.

对３１例原发性肝癌患者血清进行巢式聚合酶链反应扩增ＨＢＶ一ＤＮＡ，并对限阳性者之Ｃ基因前Ｃ区终２８、Ｃ区Ｌ９７位点进行限制性酶切片段长度多态性分析，同时与无症状ＨＢＶ携带者９例、慢性活动性乙型肝炎１４例对照。结果显示：３１例肝癌患者中１１例ＨＢＶ一ＤＮＡ阳性，其中终２８变异２例，Ｌ９７变异５例。研究认为一部分肝癌患者不仅存在病毒复制，且与对照组一样有变异毒株存在。

erum HBV DNA was detected with nested-PCR in 3l patients with hepatocellular carcinoma, and thevariations of stop 28 at prec and L97 at C gene were tested in the positives with restriction fragment lengthpolymorphism analysis。 Fourteen cases with chronic active hepatitis B and nine asymptomatic HBV carrierswere examined for comparison。 As a result, 11 out of 31 cases of hepatocellular carcinoma were sero-positive,of HBV DNA, in which five cases with L97 variation and two with stop 28 were foun,respectively。 Com-paratively, of fourteen cases with chronic active hepatitis ten cases for each of stop 28 and L97 substitutionwere found respectively ; in nine cases of asymptomatic HBV carriers four were found with stop 28 and twowith L97 substitution。 Therefore, in a portion of patients with hepatocellular carcinoma not only viremia ofHBV DNA can be detected , but also the viruses were variate at site stop 28 as well as L97。