HBsAg阴性慢性HBV感染：S基因a表位未发现变异

NO MUTATI0N AT “A”EPIT0PE OF S GENE IN CHR0NIC HEPATITIS B VIRUS INFECTI0N WITH HBsAg NEGATIVE

聚合酶链反应可检出ＨＢｓＡｇ（一）的乙型肝炎病毒感染，流行率在健康人群为１．９％、２．０％、３．４％，献血员中２．８％，在输血后急性丙型肝炎中合并３４．０％，在急性起病的“非甲非乙”病毒性肝炎中４７．２％，在慢性活动性“非甲非乙”肝炎中６６．７％，在重型乙型肝炎中３８．７％，在ＨＢｓＡｇ（一）原发性肝癌中８３．３％。对１１例各类病人扩增其血清中ＨＢＶＤＮＡ／Ｓ基因，对编码ａ表位的区段序列分析，未发现变异。

HBsAg negative HBV infections were detected with polymerase chain reaction (PCR). The positive ratewere l.9%,2.0%and 3.4%in three healthy groups,.8%in blood donors, 34.0% coinfected in acutepost-transfusion hepatitis C, 47.2%in acute onset“non-A, non-B” hepatitis, 66.7%in chronic “non-A, non-B” hepatitis, 38.7%in liver failure of hepatitis B,and 83.3%in primary liver cancer negative for HBsAg。The S gene of HBV DNA in 11 sera of various categories of above patients was amplified with PCR, and theregion encoding“a”epitope was sequenced. No mutation was found. Accordingly, the causes of HBsAg nega-tivity were discussed suspectively。