血液制品中丙型肝炎病毒状况的初步研究

PRELIMINARYSY STUDY OF HCV CONTAMINATION IN BLOOO PRODUCTS

利用第二代抗ＨＣＶＥＬＩＳＡ试剂和ＲＴ－ｎｅｓｔｅｄＰＣＲ技术对１１０份不同生产厂家和／或批号的市售血液制品（丙种球蛋白４７份，白蛋白２７份、纤维蛋白原２份，Ⅷ因子３份，白细胞和血小板悬液血浆３１份）进行了抗ＨＣＶ和ＨＣＶＲＮＡ检测，结果４７份丙种球蛋白均为抗ＮＣＶ阳性，其中１５份（３１．９ｌ％）ＨＣＶＲＮＡ阳性；１份纤维蛋白原抗ＨＣＶ和ＨＣＶＲＮＡ阻性；白蛋白。Ⅷ因子以及白细胞和血小板悬液血浆均为抗ＨＣＶ阴性，但分别有２份（７．４１％）０份和２份（６．４５％）ＨＣＶＲＮＡ阳性。该结果初步说明，多种类型市售血液制品均存在ＨＣＶ污染，输注污染的血液制品存在传播ＨＣＶ感染的潜在危险性。因此在严格筛选献者同时，应进一步研究血液制品制备过程中灭活病毒问题，以确保安全使用血液制品。

ne hundredand ten sarnples of various kinds of commercial blcodproducts(including 47immunoglobulin, 27 albumine,2 fibrinogen, 3 factorⅧand 3l leucocyt-blood platelet suspension ) of differentmanufacturters an/or different branches were tested for anti-HCV and HCV RNA with the esecond generationanti-HCV ELISA kits and reverse transcription nested Polynlerase cliain red,ction(RT-nested PCR). The re-suIt showed, that all 47 sarnples of immunoglobulin were anti-HCV positive, and 15 of them were HCV RNApositive, One of fibrogen sample was anti-HCV and HCV RNA positive All samples of albumine, factor Ⅷ andleucocyt-blood platelet suspension were anti- HCV negatlve, of them 2,0, 2 samples were HCV RNA positiverespectively. This demonstrated that HCV contaminaled biood productsxtill existed. For the safty of blood-products, ef ficient vir1is inactivation procedure during their production should be consdered, except srictlyscreening of bood-donors with sensitive and reliable kits to detect anti-HCV。