66例慢性非甲非乙型肝炎患者的丙型肝炎病毒感染研究

HEPATITIS C VIRUS INFECTION IN 66 CASES OF PATIENTS WITH CHRONIC NON-A,NON-B HEP-ATITIS

以ＨＣＶ－Ｔ３序列为引物，结合ＲＴ－ＰＣＲ和寡聚核甘酸探针Ｓｏｕｔｈｅｒｎ杂交，检测６６例慢性非甲非乙型肝炎（ＮＡＮＢＨ）患者的血浆ＨＣＶ－ＲＮＡ，阳性４２例（６３．６％）。同样病例以相当于ＨＣＶＣ区基因编码和ＮＳ３区编码的人工合成肽抗原检测抗ＨＣＶ，阳性４９例（７４．２％）。这６６例慢性ＮＡＮＢＨ病例，抗ＨＣＶ和ＨＣＶ－ＲＮＡ双阳性者３８例（５７．６％）；抗ＨＣＶ阴性而ＨＣＶ－ＲＮＡ阳性者４例（６．１％）；抗ＨＣＶ阳性而ＨＣＶ－ＲＮＡ阴性者１１例（１６．７％）。其中诊断为散发型ＮＡＮＢＨ者３５例，检出ＨＣＶ－ＲＮＡ者１７例（４８．６％），为输血后ＮＡＮＢＨ者３１例，检出ＨＣＶ－ＲＮＡ者２５例（８０．７％）。

A reverse transcription-polymerase chain reaction(RT-PCR) combined with Southern hybridization was established to detect HCV-RNA in plasma with 4 nested oligonucleotides as the primers and a 5’-end labelled 32p-oligonucleotide as the prode,all of which are derived from HCV-13, a partial sequence of the NS 4 region of Taiwan strain HCV genome. Two synthesized poly-peptides corresponding to the protiens encoded by HCV-Cgene and NS3gene were also used as the antigens in ELISA for detecting serum anti-HCV.OF 66 patients with chronic non-A,non-B hepatitis,42(63.6%)were HCV-RNA positive and 49 (74.2%)anti-HCV posi-tive,4(6.1%)HCV-RNA positive but anti-HCV negative,11(16.7%)HCV-RNA negative but anti-HCV positive. 17 (48. 6%)of 35 cases diagnosed as sporadic non-A,non-B hepatitis were HCV-RNA positive, 25(80.7%)of 31 cased diagnosed as post-transfusion non-A, non-B hepatitis were HCV-RNA positive.