缓慢成孔型磷酸钙复合材料的性能研究

Study on performance of gradually hole-forming calcium phosphate polymer

目的制备并检测CPC-PLGA复合物,为骨组织工程研究提供新的支架材料.方法制备CPC-PLGA复合物,筛选最佳配比并检测其性能;将人骨髓间充质干细胞种植于CPC-PLGA复合物上并体外培养,然后进行扫描电镜观察.结果CPC-30%vol%PLGA力学性能最好,抗压强度40.5MPa,弹性模量13.8Gpa;模拟体液下降解6周材料形成100～300μm相互连通孔隙;骨髓间充质干细胞在支架材料表面生长良好,并通过降解产生的孔洞内材料深部长入.结论CPC-PLGA复合支架材料具有优良特性,适于骨组织工程支架.

Objective Preparing and testing CPC-PLGA polymer tissue engineered bone ,providing a new-type scaffold material for Bone Tissue Engineering.Methods Prepared CPC-PLGA polymer,choosed the best proportion and tested its performance; Human bone marrow mesenchymal sten cells(BMSCs) were seeded into CPC-PLGA polymer and cultured in vitro,then detected by SEM.Results CPC-30%vol%PLGA had the best mechanical capability,σ=40.5 Mpa,Ε =13.8 Gpa;Intercommunicative holes which size was among 100 μm～300 μm was observed in polymer after its degradation in simulative body fluid for 6 weeks; BMSCs could grow on the material surface well,moreover develop toward interior via the hole formed with degradation.Conclusion Fine performance was found in CPC-PLGA polymer and this material could be made for bone tissue engineering scaffold.