冠心病猝死心肌mcl-1蛋白检测及其意义

An immunohistochemical study on changes of mcl-1 in cases of sudden coronary death

目的观察冠心病猝死(SCD)心肌mcl-1蛋白产物,探讨其免疫组化检测及其对SCD诊断的意义。方法运用免疫组织化学SABC法,对46例SCD和40例非猝死心肌(有冠心病和无冠心病)中mcl-1蛋白产物进行检测和观察,并比较其差异。结果(1)自症状出现至死亡,时间超过30min的SCD(36例),其心肌组织均出现mcl-1蛋白阳性染色;(2)自症状出现至死亡,时间短于30min的SCD(10例),其心肌组织mcl-1蛋白呈弱阳性染色;(3)冠心病非猝死样本(20例),4例心肌出现微弱的mcl-1蛋白阳性染色,无冠心病非猝死样本(20例)几乎没有出现阳性染色。结论心肌mcl-1蛋白的免疫组化检测可诊断自症状出现至死亡时间超过30min的SCD。

Objective To investigate changes of mcl-1 protein in cases of sudden coronary death (SCD) and applicability of mcl-1 protein to diagnosis of SCD by immunohistochemical technique. Methods 46 cases died of SCD were selected and 40 cases died of unnatural causes with or without coronary heart disease were used as control. mcl-1 protein was detected by immunohistochemical SABC method. Results (1)mcl-1-positive cardiac cells was detected in 36 cases of SCD who died over 30 minutes from the onset of symptoms; (2) A weak positive immunostaing of the protein was observed in myocardial fibers in 10 cases of SCD whose death occurred less than 30 minutes from the onset of symptoms. (3) Of 40 control cases who died of unnatural causes, 4 of 20 cases with severe coronary heart disease showed a feeble mcl-1 positive staining, and the other 20 control cases without coronary heart disease revealed almost no mcl-1-positive staining in myocardium. Conclusion The immunohistochemical detection of mcl-1 protein can be used as a diagnostic marker for identification of the cases who died of SCD over 30 minutes from the onset of symptoms.