非小细胞肺癌组织中内皮抑素mRNA的转录表达及临床意义

Transcription expression of endostatin mRNA in non-small cell lung cancer tissues

背景与目的肿瘤生长和转移依赖于肿瘤血管形成,抑制其血管形成能够控制其生长和转移。胶原ⅩⅧ/内皮抑素(collagenⅩⅧ/endostatin)是迄今为止最有效的内源性血管生长抑制剂之一。本研究拟探讨内皮抑素mRNA在非小细胞肺癌组织中的转录表达及其与肺癌临床病理生理特征的关系。方法用RTPCR法检测46例非小细胞肺癌组织和14例肺良性病变组织中内皮抑素mRNA的转录表达。结果①肺癌组织中内皮抑素mRNA转录表达水平(0.872±0.071)显著高于癌旁肺组织(0.717±0.073)和肺良性病变肺组织(0.611±0.026)(P<0.001),癌旁肺组织亦显著高于肺良性病变肺组织(P<0.01)。②肺癌组织中内皮抑素mRNA转录表达水平与肺癌原发肿瘤大小、有无远处转移、细胞分化程度和PTNM分期等均有密切关系,而与肺癌原发部位、淋巴结转移状态、组织学类型,患者性别、年龄和吸烟与否等均无明显关系。结论肺癌患者肺癌组织中存在内皮抑素mRNA转录表达水平的升高,这种升高与肺癌的发生、发展、远处转移和细胞分化不良有密切关系,因而有助于预测肺癌的恶性行为。

Background and objective It has been proved that tumor development and metastasis are dependent on angiogenesis. Suppression of tumor angiogenesis can inhibit tumor growth and metastasis. Collagen ⅩⅧ/endostatin is one of the most effective inhibitors of angiogenesis at present. The aim of this study is to study the relationship between transcription expression of endostatin mRNA and clinical and pathophysiological characteristics in non-small cell lung cancer (NSCLC). Methods The transcription expression of endostatin mRNA was detected in 46 lung cancer tissues and paracancerous lung tissues, 14 benign pulmonary lesion tissues as control by RT-PCR method. Results ①The transcription expression of endostatin mRNA in lung cancer tissues (0.872±0.071) was significantly higher than that in paracancerous tissues (0.717±0.073) and benign pulmonary lesion tissues (0.611±0.026) (P<0.001).②The transcription expression of endostatin mRNA in lung cancer tissues was closely related to P-TNM stages, distant metastasis, grade of cell differentiation and size of the primary tumors (P<0.05), but not to location of tumor, lymph node status, histological classification, age and sex of the patients and smoking or not (P>0.05). Conclusion The transcription expression of endostatin mRNA in NSCLC tissues is significantly higher than that in paracancerous tissues and benign pulmonary lesion tissues, and is closely related to P-TNM stages, distant metastasis, grade of cell differentiation and size of the primary tumors, hence it might be helpful to evaluate the biological behavior of lung cancer.