血管紧张素转换酶基因多态性与肺癌关系的研究

Analysis of the relationship between polymorphism of angiotensin-converting enzyme gene and lung cancer

背景与目的血管紧张素转换酶(ACE)是RAS系统的关键酶,在肺内表达丰富。近年来的研究发现ACE有着广泛的生理作用,包括参与肿瘤发生和发展的病理过程。本研究拟探讨ACE基因内一287bp的片段插入(insertion,I)与缺失(deletion,D)多态性是否与肺癌的易感性、病理类型和临床分期存在相关性。方法从47例肺癌患者和54例正常对照者的外周静脉血中提取DNA,采用PCR方法测定基因型。结果肺癌组中II、ID和DD基因型频率分别为0.447、0.447和0.106,I和D等位基因的频率分别为0.670和0.330;正常对照组中II、ID和DD基因型频率分别为0.370、0.556和0.074,I和D等位基因的频率分别为0.648和0.352,两组间各基因型和等位基因的分布无显著统计学差异(P>0.05)。小细胞与非小细胞肺癌患者间以及各临床分期间也未发现ACE基因型和等位基因分布的差异(P>0.05)。结论本研究未发现ACE的基因多态性与肺癌的易感性、病理类型或临床分期存在相关性。

Background and objective Angiotensin-converting enzyme (ACE), the key enzyme of RAS, is expressed in abundance in lung. Recent studies show that it plays various biological roles and even contributes importantly to the carcinogenesis of malignant tumors. The aim of this study is to investigate whether the insertion (I)/deletion (D) polymorphism of ACE is related to the susceptibility, pathological type or staging of lung cancer. Methods DNA was extracted from the peripheral veinous blood derived from 47 lung cancer patients and 54 normal persons. The ACE genotype of each sample was amplified with PCR. Results The frequencies of II, ID and DD genotype of ACE in lung cancer group were 0.447, 0.447 and 0.106 respectively, and the frequencies of I and D allele were 0.670 and 0.330 respectively. The frequencies of II, ID and DD geno-(type) of ACE in control group were 0.370, 0.556 and 0.074 respectively, and the frequencies of I and D allele were 0.648 and 0.352 respectively. There was no significant difference of ACE genotypes or alleles frequencies between the lung cancer and control groups. No significant difference of ACE genotypes or alleles frequencies was found between small cell lung cancer and non-small cell lung cancer groups or between the different staging groups of lung cancer patients. Conclusion No association is found between the polymorphism of ACE gene and the susceptibility, pathological type or clinical staging of lung cancer.