沐舒坦针剂治疗兔创伤性急性肺损伤的实验研究

Study of mucosolvan treatment of traumatic ALI in rabbits

目的 在兔创伤性肺损伤早期应用盐酸氨溴素 (沐舒坦 ) ,观察其对兔创伤性急性肺损伤的疗效并探讨其作用机制。方法 将实验动物 (新西兰兔 )随机分为 3组 :Ⅰ组 :对照组 (n =12 ) ;Ⅱ组 :常规剂量沐舒坦治疗组 ,静脉注射沐舒坦 5mg/kg(n =12 ) ;Ⅲ组 :大剂量沐舒坦组 ,静脉注射沐舒坦 15mg/kg (n=12 )。 3组动物均采用自行研制的BIM Ⅵ型生物撞击机撞击动物右侧胸壁致伤。分别于伤前和伤后 1、3、7小时记录动物的心率、呼吸频率的变化 ,并通过静脉插管抽血以测定血气、肿瘤坏死因子 -α(TNF α)、白细胞介素 - 1(IL 1)、IL 6、IL 8等指标 ,并进行肺组织的病理学检查。结果 实验动物肺损伤发生率达 10 0 %(36 / 36 )。对照组动物 (Ⅰ组 )伤后PaO2 明显低于Ⅱ组和Ⅲ组 (P <0 .0 5 ) ,PaCO2 明显高于Ⅱ组和Ⅲ组 (P <0 .0 5 )。Ⅱ、Ⅲ组间PaO2 、PaCO2 比较无显著性差异。Ⅱ、Ⅲ组外周血促炎因子TNF α及IL 1、IL 6、IL 8含量显著低于对照组 (P <0 .0 5 ) ,Ⅲ组血浆炎性细胞因子水平低于Ⅱ组血浆炎性细胞因子水平 (P <0 .0 5 )。组织病理学检查 :Ⅱ、Ⅲ组动物肺组织水肿、灶性出血、肺不张等急性肺损伤的表现较Ⅰ组明显减轻 ,而Ⅲ组的上述急性肺损伤的表现相对Ⅱ组有所减轻。

Objective To investigate the effects of mucosolum on acute lung injury after trauma in rabbits and its mechanism.Methods Thirty-six rabbit were divided randomly into 3 groups.Group Ⅰ:control group,12 rabbits;Ⅱ:normal dosage mucosolvan group,12 rabbits;Ⅲ:large dosage mucosolvan group,12 rabbits.The model of thoraco-abdominal impact injury in rabbits was established with a steel ball falling down to the xiphoid process of animals.Mucosolvan was injected with a dose of 15 mg/kg.Preparation of the rabbits and the wounding methods were the same.ResultsThe morbidity of acute lung injury is 100%(36/36).In group Ⅰ,PaO 2 decreased sharply after trauma.In group Ⅱ and Ⅲ,the decrease of PaO 2 were less serious than that of group Ⅰ.The content of TNF-α,IL-1,IL-6 and IL-8 in group Ⅰ was more than those of group Ⅱ and Ⅲ(P<0.05).The content of TNF-α,IL-1,IL-6 and IL-8 in group Ⅲ was less than that of group Ⅱ(P<0.05).In group Ⅱ and Ⅲ,pathologic changes of ALI such as pulmonary edema,focal hemorrhage,atelectasis were less serious than that of group Ⅰ.In group Ⅲ,those changes were less serious than that of group Ⅱ.Conclusion Mucosolvan has definite treatment effects on traumatic ALI.