载脂蛋白E基因多态性与颅脑损伤的相关性

A study on the correlation between the apolipoprotein E polymorphism and traumatic brain injury

目的探讨在中国人群中载脂蛋白E(APOE)基因多态性与颅脑损伤病情的相关性。方法收集70例颅脑损伤患者的临床资料,每对象抽取静脉血2ml,提取基因组DNA,采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)检测APOE基因型,分型结果与临床资料等分别采用SPSS11.5软件进行χ2检验和Logistic回归分析。结果在13例APOEε4等位基因携带者中有5例(38%)病情加重(较入院时脑内血肿体积增大,迟发性血肿等),明显高于非ε4等位基因携带者(12%,P<0.05);单因素和多因素logistic回归分析均提示,ε4是病情加重的危险因素。结论APOEε4等位基因是颅脑损伤患者病情加重的危险因素。

Objective To determine the relationship between apolipoprotein E (APOE) polymorphism and the patient’s condition after traumatic brain injury in a cohort of Chinese patients. Methods Venous blood was collected at the time of admission to determine the APOE genotype in 70 Chinese patients who presented with traumatic brain injury. APOE polymorphism was determined by PCR-RFLP. X2 test and logistic regression analyses via SPSS version 11.5 were performed to analyze the genotype and clinical data. Result In 70 Chinese patients,the distributions of APOE genetypes and alleles matched Hardy-Weinberg Law. Five (38%) out of 13 patients with APOEε4 had an deteriorated condition (with hematoma enlarged compared that at admission or hematoma delayed), which was significantly different from seven (12%) out of 57 patients without APOEε4 (P<0.05). Logistic regression analyses showed that APOEε4 was a risk factor to injury aggravation after traumatic brain injury. Conclusion APOEε4 is the risk factor to injury aggravation after traumatic brain injury.