磁共振血管成像评估兔创伤性肢体深静脉血栓:与金标准的一致性

Magnetic resonance angiography for evaluation of rabbit's deep venous thrombosis in traumatic limbs:coincidence with golden standard

目的:通过与金标准数字减影血管造影成像比较,探讨磁共振血管成像对兔创伤性肢体深静脉血栓的诊断价值。方法:实验于2003-10/2004-03在昆明医学院外科总论实验室完成。新西兰大白兔6只随机分为3组,每组2只。采用自制击打装置,建立兔骨折和创伤模型。骨折固定组兔造模后骨折,创伤固定组兔造模后不骨折,单纯固定组兔不造模,3组均行石膏外固定。于处理后7d各组兔分别用磁共振血管成像及数字减影血管造影对兔左股静脉血栓进行检测,并进行组织学观察。结果:3组6只兔均进入结果分析。①磁共振血管造影和数字减影血管造影检测:第7天骨折固定组和创伤固定组各2只血栓阳性,单纯固定组未发生。②组织学观察:骨折固定组和创伤固定组第7天均可见股静脉血栓形成,单纯固定组均未见血栓形成。结论:磁共振血管造影与数字减影血管造影在深静脉血栓形成诊断方面有较好的一致性、准确性和可靠性,磁共振血管造影可作为临床检测深静脉血栓的另一种检查手段。

AIM: To investigate the diagnostic value of magnetic resonance angiography (MRA) on the deep venous thrombosis (DVT) in traumatic limbs of New Zealand white rabbits through the comparison between MRA and golden standard digital substraction angiography (DSA). METHODS: The experiment was completed at the Surgical Laboratory of Kunming Medical College from October 2003 to March 2004. Six New Zealand white rabbits were randomly divided into 3 groups with 2 in each. The animal models of fracture and trauma were established with self-made instrument. Rabbits in fracture group were fracture after sample preparation, but rabbits without fracture in trauma group and without sample preparation in simple fixation group. Rabbits in all three groups were fixed with plaster extermally. Left DVT of rabbit was assayed with MRA and DSA days after treatment, then the histological observation was used. RESULTS: All 3 rabbits in the 3 groups entered the final analysis. ①On the seventh day, two positive thrombuses in fracture group and trauma group respectively were detected with MRA and DSA, but none in simple fixation group. ②Histological observation: On the seventh day, femoral venous thrombosis was observed in fracture group and trauma group respectively, but none in simple fixation group. CONCLUSION: It is coincident, exact and reliable for MRA and DSA to diagnosis DVT, and also MRA may be used as an available method in the clinical DVT diagnosis.