鲍曼不动杆菌β内酰胺酶基因表达和对抗生素的耐药性研究

Study on the drug resistance and gene-expression of β-lactamases in Acinetobacter baumanii

目的:调查淮南地区临床分离的鲍曼不动杆菌耐药性及β内酰胺酶基因型。方法:采用K-B法测定临床分离的32株鲍曼不动杆菌对14种抗菌药物的敏感性,采用聚合酶链反应(PCR)及序列分析的方法分析β内酰胺酶基因型。结果:鲍曼不动杆菌对亚胺培南(100%)、头孢吡肟(87.5%)、阿米卡星(87.5%)和舒普深(84.4%)敏感,对其他抗菌药物的耐药率均较高。β内酰胺酶基因型分布TEM-1阳性率100%(32/32),SHV-12阳性40%(12/32),OXA-2阳性12.5%(4/32),CTX-M各型均阴性,VEB和PER均阴性。结论:淮南地区临床分离的鲍曼不动杆菌耐药严重,至少存在3种不同类型的β内酰胺酶,基因型分别为TEM-1、SHV-12、OXA-2。

Objective:To investigate the resistance and gene-expression of β-lactamases in Acinetobacter baumanii isolated from huai-nan area.Methods:K-B tests were performed to detect the susceptibility of 14 kinds of antimicrobial agents against 32 strains of Acinetobacter baumanii gene-expression of β-lactamases was analyzed by PCR and verificated by DNA sequencing.Results:The highly sensitive rates of 32 strains to Imipenam, Cefepime, Amikaxin and Sulperazon were 100%, 87.5%,87.5% and 84.4%, respectively. The others have highly resistance rates.β-lactamases genotype were positive distributed, the positive rate of TEM-1, SHV-12, OXA-2 was 100%(32/32),40%(12/32),12.5%(4/32) respectively.The other strains (CTX-M,VEB and PER) were negative.Conclusion:The resistance of Acinetobacter baumanii isolated from Huai-nan area was a serious issue and at least 3 kinds of β-lactamases exsisted including TEM-1,SHV-12 and OXA-2.