狂犬病快速诊断方法的建立和应用

Development and application of rapid diagnosis for rabies virus

根据狂犬病病毒核蛋白基因保守区序列设计1对引物,建立了用于狂犬病病毒特异性核酸检测的RTPCR技术。该技术可从狂犬病病毒CVS株、8202株和SRV9株的含毒细胞培养物及鼠脑组织中,扩增出443bp的核酸片段,检出核酸的敏感性约为3pg。对30份不同种动物脑组织的检测结果与小鼠脑内接种试验(MIT)的测定结果完全吻合,但前者可在3h内直接对组织匀浆进行诊断,具有快速。

A reverse transcription and polymerase chain reaction (RT-PCR) technique for viral nucleic acids has been developed as an alternative protocol for diagnosis of rabies virus.A primer set mapping in the nucleoptotein cistron allowed a specific and sensitive amplification of infected animal brain materials and tissue-culture supernatants.30 brain samples from different animal species including 12 dogs,10 mice and 8 cattle,4 deer,2 horses,which were found to be positive by means of the mouse inoculation test (MIT), were retested with the RT-PCR.Compared with FAT and MIT ,RT-PCR was more rapid and efficient than the MIT to detect rabies virus.