葛根中有效部位及有效成分的高效液相色谱分析

Qualitative and quantitative analysis of the effective part and effective components of Radix Puerariae

目的建立全面控制葛根及其制剂的定性和定量分析方法。方法用反相高效液相色谱法(RPHPLC),以甲醇水(42∶58)为流动相在250nm波长下检测,对葛根有效部位(醋酸乙酯提取部位)进行指纹图谱定性分析,对葛根有效部位中的有效成分葛根素和大豆苷元进行定量测定,并对11种不同来源的葛根药材进行分析测定。结果在定性分析条件下,葛根有效部位的HPLC指纹图谱中有5个共有峰,可以作为其定性鉴别的指标峰;在以葛根素和大豆苷元为定量指标分析中,其方法的回收率分别为98.0%和95.4%,相对标准偏差(RSD)分别为1.30%和1.61%,葛根药材中葛根素和大豆苷元的含量分别在0.213%～3.626%和0.012%～0.049%范围内。结论以葛根有效部位指纹图谱定性分析和有效成分定量分析方法,可以准确有效地控制其质量。

Objective To establish a qualitative and quantitative method for controlling the quality of Radix Puerariae and its preparation. Methods The qualitative fingerprint of effective part (extract part of ethyl acetate) was obtained by using RP-HPLC with methanol-water (42∶58) as mobile phase and UV detection at 250 nm . The quantitative measurement of effective components (puerarin and daidzein) was finished under the above chromatographic conditions. And the 11 kinds of different samples of Radix Puerariae were analyzed by the method. Results Under the qualitative conditions, the 5 common peaks in RP-HPLC fingerprint of the effective part could be used as index peaks for qualitative identification. In RP-HPLC quantitative analysis, the recoveries of the method were 98.0% with RSD of 1.30% for puerarin and 95.4% with RSD of 1.61% for daidzein, respectively. The amount of puerarin and daidzein in Radix Puerariae was 0.213%～3.626% and 0.012%～ 0.049% , respectively. Conclusion The analytical method with qualitative fingerprint of effective part and quantitative measurement of effective components of Radix Puerariae can be accurately used for controlling its quality.