生长抑素受体家族介导的子宫内膜癌细胞系HEC-1A的生长抑制作用

The growth-inhibitory effect of endometrial carcinoma cell line HEC-1A induced by somatostatin receptor family

目的研究生长抑素受体家族在子宫内膜癌细胞系HEC1A上的表达,并应用生长抑素类似物RC160活化生长抑素受体,观察子宫内膜癌细胞系HEC1A的生长及凋亡。方法利用RTPCR检测HEC1A细胞上的生长抑素受体。BrdU整合试验测定RC160对HEC1A细胞的抗增殖作用。TUNEL染色检测是否存在细胞凋亡。结果所有5种生长抑素受体亚型在HEC1A细胞中均有表达。RC160可抑制HEC1A细胞的生长,并具有剂量依赖性特点。在处理48h后,10-5mol/L浓度的RC160达到最大抑制效应。未检测到明显的细胞凋亡。结论生长抑素类似物RC160通过结合HEC1A生长抑素受体而抑制细胞增殖。细胞凋亡在此抑制过程中不起主要作用。

Objective To study the expression of somatostatin receptor family in endometrial carcinoma cell line HEC-1A, as well as the growth and apoptosis of HEC-1A cell line when it was treated with somatostatin analogue RC-160. Methods RT-PCR was used to examine the existence of somatostatin receptors on the HEC-1A cells. The anti-proliferative effect of RC-160 on the growth of HEC-1A cells was detected by using BrdU incorporation test. TUNEL staining was used to find out whether apoptosis was involved in the inhibitory process. Results All the five somatostatin receptor subtypes were expressed in HEC-1A cells. RC-160 inhibited the HEC-1A cell growth stimulated by serum in a dose-dependent manner. The effect was maximal at the concentration of 10 -5 mol/L after 48 hours' treatment. No apoptosis was detected. Conclusion Somatostatin analogue RC-160 can inhibit the proliferation of endometrial carcinoma cell line HEC-1A through binding to the somatostatin receptors on the cells. It seems that apoptosis is not the major cause of the inhibition.