奋乃静对K562白血病细胞的诱导分化作用

Differentiation-inducing effects of perphenazine on K562 leukemia cells

目的:观察吩噻嗪类抗精神病药奋乃静对K562白血病细胞的诱导分化作用。方法:采用液体培养实验,MTT实验,集落培养实验观察奋乃静对K562细胞增殖能力的影响,采用WG染色观察细胞形态学变化和流式细胞术检测膜CD71分化抗原观察K562细胞功能变化三个方面评价奋乃静对K562细胞的诱导分化作用。结果:吩噻嗪类抗精神病药奋乃静可上调K562细胞膜CD71分化抗原的表达量,升高细胞内Hb含量,抑制K562细胞增殖并在形态上有趋向分化的改变。结论:奋乃静有诱导K562细胞分化的作用。

Objective To determine the differentiation-inducing effects of perphenazine on K562 leukemia cells. Methods Differentiation-Inducing effects of a phenothiazine perphenazine were evaluated by proliferation, morphology and function of K562 cells.We evaluated the effects of perphenazine on K562 cells proliferation by cellular enumeration in liquid culture assay, MTT assay and clony formation assay, the morphology by Wight-Gimesa staining, and the function by detecting CD71 through flow cytometry . Results Perphenazine enhanced the expression of CD71 on K562 cells and increased Hb content in K562 cells, while inhibited the proliferation of K562 cells. K562 cells showed differentiation morphology after the drug treatment. Conclusion Perphenazine possessed differentiation-inducing effects on K562 cells.