微囊化Maspin基因修饰细胞对乳腺癌细胞Bcap37运动及黏附功能的影响

Effects of microencapsulated CHO cells modified with maspin gene on the motility and adhesiveness of breast carcinoma cells Bcap37

目的探讨Maspin在抗肿瘤转移过程中的作用及微囊化基因修饰细胞治疗恶性肿瘤的可能性和可行性。方法将微囊化Maspin基因修饰的中国仓鼠卵巢上皮细胞(CHO细胞)与乳腺癌细胞Bcap37进行共同培养,观察Bcap37细胞运动功能的变化;观察Bcap37细胞对同质细胞(Bcap37)和异质细胞(人血管内皮细胞ECV204)黏附功能的变化;同时观察被干预后的Bcap37细胞黏附分子CD44v6和E2Cadherin表达的改变。结果经微囊化Maspin基因修饰的CHO细胞处理后的Bcap37细胞,其迁徙功能明显减弱,对Bcap37细胞同质黏附功能明显增强,对ECV204细胞异质黏附功能明显减弱;经微囊化CHO/Maspin细胞处理后,Bcap37细胞的黏附分子E2Cad表达增强,而CD44v6表达有所降低。结论微囊化Maspin基因修饰的CHO细胞对乳腺癌细胞Bcap37的运动和黏附功能有一定影响;Maspin通过抑制肿瘤细胞的运动功能、异质黏附功能和增强同质黏附功能抑制肿瘤细胞的转移;微囊化基因修饰细胞可以用于治疗恶性肿瘤。

Objective To investigate the effects of microencapsulated Chinese hamster ovary (CHO ) cells modified with maspin gene on the motility and adhesiveness of breast carcinoma cells Bcap37 and to explore the possibility and feasibility of its clinical application in treatment of malignant tumors. Methods After the Bcap37 cells were co cultured with the microencapsulated CHO cells modified with maspin gene, their motility and adhesion to vascular endothelial cells (ECV304), changes in CD44v6 and E cadherin expression were examined. Results After the treatment, the motility of Bcap37 cells, their adhesion to vascular endothelial cells ECV304 and the CD44v6 expression were significantly reduced. The adhesiveness of Bcap37 cells and their E cadherin expression were significantly enhanced. Conclusion The microencapsulated CHO cells modified with maspin gene decrease motility and adhesiveness of breast carcinoma cells Bcap37, which help explain the anti metastatic effects of maspin.