14-3-3ζ抑制肺癌细胞转移的实验研究

Inhibition of metastasis of lung cancer cells by 14-3-3ζ protein:an experimental investigation

目的:探讨14-3-3ζ与肺癌转移的关系。方法:以人肺巨细胞癌高低转移细胞株为模型,Western印迹验证14-3-3ζ在高低转移株中的差异表达;随后采用细胞转染技术将1433ζ基因导入人肺巨细胞癌高低转移株,通过研究转染后细胞增殖能力、黏附能力以及迁移能力的变化,探讨14-3-3ζ与肿瘤转移的相关性。结果:Western印迹检测发现14-3-3ζ在低转移株PLA801C中的表达水平显著高于高转移株PLA801D,在此基础上,构建成功正反义表达载体并转染细胞,转染正义载体后细胞的增殖减慢,黏附能力提高,体外迁移能力下降;而转染反义载体后细胞的增殖加快,体外迁移能力提高。结论:1433ζ可能抑制肺癌细胞的转移。

Objective: To investigate the relation between 14-3-3ζ and lung cancer metastasis. Methods:The differential expression of 14-3-3ζ between highly(PLA-801D) and poorly(PLA-801C) metastatic sublines of human lung giant cell carcinoma was examined by Western blot. The sense and antisense expressed vectors of 14-3-3ζ were constructed and transfected into PLA-801C and PLA-801D with Lipofectamine2000. Cell′s ability of proliferation, adhesion and migration after transfection was studied. Results: Western blot exmination found that 14-3-3ζ expressed much more highly in PLA-801C than in PLA-801D. After transfected with the sense expression vector, cells displayed lower proliferation ability, higher adhesion ability and lower migration ability as compared with cells transfected with mock control; while after transfected with the antisense expression vector, cells showed higher proliferation ability and migration ability. Conclusion: 14-3-3ζ might inhibit the metastasis of lung cancer cells.