摘要
目的:研究皮肤组织在mRNA及蛋白水平表达大麻CB2受体的情况。方法:RTPCR和免疫印迹方法研究CB2受体在大鼠皮肤的表达,免疫组化方法示CB2受体的组织分布。结果:RTPCR方法可检测到大鼠皮肤、脾脏组织中CB2受体在mRNA水平的表达;Western印迹示大鼠皮肤、脾脏组织匀浆中抗CB2抗体,可检测到41.6×103蛋白带,而同为实质脏器的肝脏无CB2受体表达;免疫组化方法证实CB2受体主要分布于皮肤的表皮组织和真皮毛囊组织。结论:CB2受体在皮肤组织mRNA和蛋白水平均有表达,主要分布于表皮和毛囊组织。CB2受体可能是神经系统调节皮肤功能的桥梁。
Objective: To study the expression and distribution of CB2 in rat skin tissue. Methods: The expression of CB2 in rat skin was examined by RT-PCR and Western blot,and the distribution of CB2 in rat skin tissue by immunohistochemistry. Results: The expression of CB2 mRNA was found in rat skin. The Western blot showed that the rat normal skin and the spleen tissue expressed CB2 at the protein level. CB(2) receptors were^( )mostly present in suprabasal layers in the epidermis and hair follicles in dermis detected by CB2-like immunoreactivity with a N-terminal anti-CB2 receptor antibody. Conclusion: The results support that the endocannabinoid system is expressed in skin tissue and suggest the involvement of CB2 receptor in the communication between central nervous system and skin.
出处
《军事医学科学院院刊》
CSCD
北大核心
2005年第3期257-259,共3页
Bulletin of the Academy of Military Medical Sciences
关键词
CB2受体
皮肤
毛囊
中枢神经系统
cannabinoid receptor CB2
skin
hair follicle
central nervous system