大鼠皮肤大麻CB2受体在mRNA及蛋白水平的表达和组织分布

Expression and distribution of CB2 (cannabinoid receptor) in rat skin

目的:研究皮肤组织在mRNA及蛋白水平表达大麻CB2受体的情况。方法:RTPCR和免疫印迹方法研究CB2受体在大鼠皮肤的表达,免疫组化方法示CB2受体的组织分布。结果:RTPCR方法可检测到大鼠皮肤、脾脏组织中CB2受体在mRNA水平的表达;Western印迹示大鼠皮肤、脾脏组织匀浆中抗CB2抗体,可检测到41.6×103蛋白带,而同为实质脏器的肝脏无CB2受体表达;免疫组化方法证实CB2受体主要分布于皮肤的表皮组织和真皮毛囊组织。结论:CB2受体在皮肤组织mRNA和蛋白水平均有表达,主要分布于表皮和毛囊组织。CB2受体可能是神经系统调节皮肤功能的桥梁。

Objective: To study the expression and distribution of CB2 in rat skin tissue. Methods: The expression of CB2 in rat skin was examined by RT-PCR and Western blot,and the distribution of CB2 in rat skin tissue by immunohistochemistry. Results: The expression of CB2 mRNA was found in rat skin. The Western blot showed that the rat normal skin and the spleen tissue expressed CB2 at the protein level. CB(2) receptors were^( )mostly present in suprabasal layers in the epidermis and hair follicles in dermis detected by CB2-like immunoreactivity with a N-terminal anti-CB2 receptor antibody. Conclusion: The results support that the endocannabinoid system is expressed in skin tissue and suggest the involvement of CB2 receptor in the communication between central nervous system and skin.