辛伐他汀对氧化型低密度脂蛋白诱导的猪冠状动脉平滑肌细胞增殖和迁移的影响

Effect of simvastatin on proliferation and migration of cultured porcine coronary smooth muscle cells induced by oxidized low density lipoprotein

目的观察辛伐他汀对氧化型低密度脂蛋白(OX-LDL)诱导的猪冠状动脉平滑肌细胞(SMC)增殖和迁移的影响。方法采用体外猪冠状动脉SMC培养技术,以3H-TdR的参入量表示冠状动脉的SMCDNA合成情况,应用划线方法测定冠状动脉SMC的迁移距离,观察辛伐他汀对OX-LDL诱导的冠状动脉SMC增殖和迁移的影响。结果浓度为10-8~10-5mol/L的辛伐他汀可使猪冠状动脉SMC的3H-TdR参入量减少,与对照组相比差异具有统计学意义;且药物浓度越高,参入量越少。10-8~10-5mol/L辛伐他汀均可使猪冠状动脉SMC的迁移距离减少,与对照组相比差异具有统计学意义。结论辛伐他汀可剂量依赖性地抑制OX-LDL诱导的猪冠状动脉SMC的增殖和迁移。

Objective To investigate the inhibition effects of simvastatin on proliferation and migration of cultured porcine coronary vascular smooth muscle cells (SMCs) induced by oxidized lower density lipoprotein (OX-LDL). Methods Porcine coronary SMCs were incubated with serum-free medium containing different concentrations of simvastatin. Infiltration of ~3H-TdR into SMC DNA was measured as an index of cell proliferation. Simvastatin's effect on migration was tested with Sarkar's technique. Results Different concentrations of simvastatin (10^(-8)-10^(-5) mol/L) caused a decrease in ~3H-TdR infiltration in porcine coronary SMC. The migration number of porcine coronary SMCs also decreased proportional to the simvastatin concentration. Conclusion Simvastatin can inhibit the proliferation and migration of porcine coronary SMCs stimulated by OX-LDL in a dose-dependent manner.