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变性梯度凝胶电泳及其在法医学中的应用 被引量：7

The forensic application of denaturalizing laddered gel electrophoresis
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作者 黄代新 王功跃
机构地区 华中科技大学同济医学院法医学系 浙江省舟山市定海区人民检察院
出处 《法律与医学杂志》 2005年第2期145-148,共4页 Journal of Law & Medicine
基金 国家自然科学基金资助(No.30300399)
关键词 变性梯度凝胶电泳 法医学应用
分类号 R919.2 [医药卫生—药学]
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参考文献22

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  • 2Fischer SG, Lerman LS. DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory[J]. Proc Natl Acad Sci USA, 1983, 80 (6):1579～1583
  • 3Sheffield VC, Cox DR, Lerman LS, et al. Attachment of a 40-basepair G + C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of singlebase changes[J]. Proc Natl Acad Sci USA, 1989, 86 (1): 232～236
  • 4Hovig E, Smith-Sorensen B, Brogger A, et al. Constant denaturant gel electrophoresis,a modification of denaturing gradient gel electrophoresis, in mutation detection[J]. Mutat Res, 1991, 262 (1): 63～71
  • 5Rosenbaum V, Riesner D. Temperature-gradient gel electrophoresis.Thermodynamic analysis of nucleic acids and proteins in purified form and in cellular extracts[J]. Biophys Chem, 1987, 26 (2-3): 235～246
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  • 7Yoshino K, Nishigaki K, Husimi Y. Temperature sweep gel electrophoresis: a simple method to detect point mutations [J]. Nucleic Acids Res, 1991, 19 (11): 3153
  • 8Chen TJ, Boles RG, Wong LJ. Detection of mitochondrial DNA mutations by temporal temperature gradient gel electrophoresis [J]. Clin Chem, 1999, 45:1162～1167
  • 9Burmeister M, diSibio G, Cox DR, et al. Identification of polymorphisms by genomic denaturing gradient gel electrophoresis: application to the proximal region of human chromosome 21 [J]. Nucleic Acids Res, 1991, 19 (7): 1475～1481
  • 10Johnson PH, Cadiou H, Hopkinson DA. Detection of the common alpha-1-antitrypsin variants by denaturing gradient gel electrophoresis [J]. Ann Hum Genet, 1991, 55 ( Pt 3): 183～198

同被引文献57

  • 1YING Yanling,LV Zhenmei,MIN Hang,CHENG Jun.Dynamic changes of microbial community diversity in a photohydrogen producing reactor monitored by PCR-DGGE[J].Journal of Environmental Sciences,2008,20(9):1118-1125. 被引量：11
  • 2Tam PK, Garcia-Barcelo M. Molecular genetics of Hirschsprung's disease. Semin Pediatr Surg, 2004,13(4): 236-248.
  • 3Miquelajauregui A, Van de Putte T, Polyakov A, et al. Smad-interacting prolein 1 (Zfhxlb) acts upstream of Wnt signaling in the mouse hippocampus and controls its formation. Proc Natl Acad Sci USA,2007, 104(31): 12919-12924.
  • 4Albano H, Henriques I, Correia A, et al. Characterization of microbial population of "Alheira" (a traditional Portuguese fermented sausage) by PCR-DGGE and traditional cultural microbiological methods. J Appl Microbiol, 2008, 105 (6) :2187-2194.
  • 5Wong ML, Medrano JF. Real-time PCR for mRNA quantitation. Biotechniques, 2005, 39(1) : 116-121.
  • 6G hring K, Mikeler E, Jahn G, et al. Rapid simultaneous detection by real time PCR of cytomegalovirus UL97 mutations in codons 460 and 520 conferring ganciclovir resistance. J Clin Microbiol, 2006, 44(12): 4541-4544.
  • 7Provenzano M, Mocellin S. Complementary techniques: validation of gene expression data by quantitative real time PCR. Adv Exp Med Biol, 2007, 593: 66-73.
  • 8Garavelli L, Donadio A, Zanacca C, et al. Hirschsprung disease, mental retardation, characteristic facial fealures, and mutation in the gene ZFHX1B(SIP1 ): confirmation of the Mowat Wilson sydrome. Am J Med Genet, 2003, 116A(4): 385-388.
  • 9Lutz S,Weisser HJ,Heizmann J,et al.Mitochondrial heteroplasmy among maternally related individuals[J].Int J Legal Med,1999,113:155-161.
  • 10Lutz-Bonengel S,Sanger T,Pollak S,et al.Different methods to determine length heteroplasmy within the mitochondrial control region[J].Int J Legal Med,2004,118:274-281.

引证文献7

二级引证文献9

法律与医学杂志
2005年 第2期

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