NS-398对骨肉瘤细胞的作用及其对Survivin表达的影响

The influence of NS-398 on induction of apoptosis and expression of survivin in osteosarcoma cells

目的:观察选择性环氧合酶-2抑制剂NS-398对人骨肉瘤细胞系MG-63的凋亡的影响及其对survivin基因表达的影响。方法:体外培养骨肉瘤细胞系MG-63,应用MTT法研究不同浓度NS-398对骨肉瘤细胞系MG-63的凋亡诱导作用,流式细胞仪检测细胞周期的改变及凋亡率的变化,RT-PCR法分析NS-398对骨肉瘤细胞系MG-63中sur-vivin基因表达的影响。结果:NS-398可诱导骨肉瘤细胞系MG-63发生凋亡,并具有剂量依赖性,在NS-398浓度为200μmol/L时,对细胞生长有明显的抑制作用。流式细胞仪检测显示NS-398引起MG-63细胞凋亡率增加。RT-PCR分析显示经NS-398作用后的骨肉瘤细胞系MG-63表达survivin较用药前明显下降。结论:NS-398对骨肉瘤细胞系MG-63有杀伤作用,其作用可能通过抑制survivin的表达,促进肿瘤细胞的凋亡而实现。

Objective:To investigate the effect of NS-398,a Cyclooxygenase-2-selected-inhibitor,on the growth inhibitory induction and apoptosis in osteosarcoma cells line MG-63 and the influence of NS-398 on the expression of survivin in MG-63 cells.Method:Osteosarcoma cells line MG-63 was cultured in vitro.Methyl thiazolyl tetrazolium(MTT)and flow cytometry(FCM)were used to study the effect of NS-398 with different concentration induced apoptosis in osteosarcoma cells line MG-63.The expression of survivin of osteosarcoma cells line MG-63 was analysed by reverse transcription-polymerase chain reaction(RT-PCR).Result:The apoptosis of osteosarcoma cells line MG-63 could be induced by NS-398,and the apoptosis rates were different with the different concentration.The apoptosis rate rised signifieantly when the concentration of NS-398 was over 200 μmol/L.The expression of survivin of osteosarcoma cells line MG-63 declined obviously than that before medication.Conclusion:NS-398 has lethal effect on osteosarcoma cells line MG-63.It may induce apoptosis of osteosarcoma cells line MG-63 by inhibiting the expression of survivin.