卡巴胆碱对脓毒症大鼠肠道功能的影响

Effects of Carbachol on intestinal barrier function in sepsis rats

目的 探讨拟胆碱药卡巴胆碱对肠缺血再灌注 (I/R)复合内毒素血症大鼠肠道功能的影响。方法 将成年雄性Wistar大鼠随机分为健康组 (N)、肠系膜上动脉夹闭 1小时后再灌注 2小时组 (I/R2 )、肠系膜上动脉夹闭后再灌注同时给卡巴胆碱 2小时组 (I/RK)、夹闭后再灌注 2小时后给内毒素组 (I/RL)和夹闭后再灌注 2小时后给内毒素同时给卡巴胆碱组 (I/RLK) ,共 5个组。分别测血中肠屏障功能指标二胺氧化酶 (DAO)、D 乳酸、D 木糖含量和肠传输。结果 I/R2 、I/RK、I/RL及I/RLK4组大鼠血浆DAO均较N组显著升高 (P <0 .0 5 ) ,但小肠DAO均较N组显著降低 (P <0 .0 5 )。I/RK和I/RLK组与I/R2 和I/RL组比较 ,前 2组血DAO显著降低 (P <0 .0 5 ) ,而小肠组织DAO显著升高 (P <0 .0 5 )。各实验组血浆D 乳酸含量较N组显著升高 (P <0 .0 5 ) ,给卡巴胆碱的I/RK和I/RLK组较I/R和I/RL组稍有降低 ,但各组间无显著性差别。各实验组小肠传输较N组显著加快 (P <0 .0 5 )。给卡巴胆碱后 ,I/RK、I/RLK组较I/R2 、I/RL组小肠传输稍减慢 ,但组间无统计学差别。I/R2 、I/RK、I/RL和I/RLK组给D 木糖 1小时后血浆D 木糖含量较N组显著升高。结论 肠局部给予卡巴胆碱对大鼠肠缺血再灌注复合内毒素所引起的肠屏障功能的损伤有一定的改善。

Objective To investigate the effects of carbachol after gut ischemia-reperfusion (I/R) combined with endtoxin challenge (I/R L ) in rats.Methods A wistar rats model of sepsis was reproduced by I/R combined with endotoxin challenge.Animals were randomly divided into normal (N)、I/R、I/R combined with carbachol(I/R K )、I/R L and I/R LK groups:various paremeters were measured in those experiments as follows:(1)Daimine oxidase activity (DAO)、D-lactate and D-xylose were measured using spectrophotometer;(2)Transit function in small intestinal.Results The results showed that plasma DAO activity significantly increased(P<0.05),and small intestinal tissue DAO activity significantly decreased (P<0.05) in I/R 2 ,I/R K ,I/R L ,and I/R LK groups as compared with those found in normal group.The level of plasma DAO was lower and small intestinal tissue DAO was higher at I/R K and I/R LK than that found in I/R and I/R L (P<0.05).Plasma D-lactate levels were markedly higher in I/R 2 、I/R K 、I/R L and I/R LK than that in N group (P< 0.05) .The concentration of plasma D-lactate was decreased at I/R K and I/R LK as compared with that at I/R and I/R L ,but there was no significant statistically difference between them.Intestinal transmission rate was significantly quicker in experiment group than in N group(P<0.05) and was slower in I/R K and I/R LK as compared with that found in I/R and I/R L ,but there was no significant statistical difference after infusion of carbachol,the Content of plasma D-xylose were increased at I/R 2 、I/R K 、I/R L and I/R KL group after infusion D-xylose(P<0.05).Conclusion The findings suggest that carbachol may be beneficial in ameliorating intestinal function after sepsis induced by ischemia-reperfusion combined with endotoxin challenge in rats.