垂体腺苷酸环化酶激活肽对大鼠创伤性脑损伤后神经细胞凋亡的影响

Effect of pituitary adenylate cyclase-activating polypeptide on apoptosis following traumatic brain injury in rats

目的观察大鼠创伤性颅脑损伤(TBI)后神经细胞凋亡现象。应用垂体腺苷酸环化酶激活肽(PACAP)观察其对损伤后大鼠神经细胞凋亡的影响。方法采用TBI模型,运用原位末端标记(Tunel)技术,观察大鼠中度脑损伤后2小时～3天伤侧大脑皮层、海马神经细胞凋亡情况。结果(1)Tunel染色:伤侧大脑半球广泛存在细胞凋亡,以受伤区周缘为甚,伤后2小时即可见凋亡细胞,2～3天达高峰。(2)PACAP治疗后12小时～3天,伤侧皮层、海马区细胞凋亡数与损伤组比较明显减少(P<0.05)。结论大鼠TBI后,神经元在发生变性、坏死的同时,存在凋亡现象。PACAP能阻滞大鼠TBI后神经细胞的凋亡,具有脑保护作用。

Objective To investigate apoptosis of neurone in rats following traumatic brain injury,and the effects of pituitary adenylate cyclase-activating polypeptide(PACAP) on apoptosis of neurone after brain injury. Methods Based on TBI animal model,the cellular apoptosis was observed in the injuried cortex and hippocampus at 2h and on 3d after moderate traumatic brain injury by TUNEL technique. Results (1) The cellular apoptosis was observed in the injuried cerebral hemisphere at 2h after traumatic brain injury,which reached its peak on 2-3d after traumatic brain injury. (2) At 12h to 3d after treatment of PACAP,the number of cellular apoptosis in the injuried cortex and hippocampus reduced significantly as compared with the controls(P<0.05). Conclusion The neuronal apoptosis exists in combination with neuronal necrosis after brain injury. PACAP can prevent the neurone from apoptosis after TBI in rats,which is of importance in the protection of brain.