甘露醇与尼莫通联合应用对大鼠脑缺血再灌注损伤保护机制的研究

Neuroprotective effects of Mannitol combined with Nimotop in treatment of ischemic reperfusion injury in rats

目的探讨甘露醇与尼莫通联合应用对大鼠局灶性脑缺血再灌注损伤的保护机制。方法SD大鼠72只随机等分成假手术组(A组)、局灶性脑缺血再灌注组(B组)、小剂量甘露醇组(C组)、大剂量甘露醇组(D组)、尼莫通组(E组)及甘露醇与尼莫通合用组(F组)6组。采用硝酸还原酶法及末端标记(TUNEL)法分别检测各组大鼠脑组织一氧化氮(NO)含量及神经细胞凋亡数目。结果大鼠脑组织NO含量及神经细胞凋亡数:(1)B组明显高于A、C、D、E及F组(P<0.05～0.01);(2)C组与D组比较差异无显著性(P>0.05);(3)F组明显低于C、D、E组(均P<0.05)。结论不同剂量的甘露醇、尼莫通及两药合用均可通过下调缺血再灌注后脑组织NO含量和减少神经细胞凋亡而发挥脑保护作用,其中两药合用效果最佳。

Objective To study neuroprotective machanisms of Mannitol combined with Nimodipine in treatment of ischemic reperfusion injury in rats. Methods Seventy-two SD rats were randomly divided into six groups (Group A:sham-operated group;Group B:model group;Group C:low-dose Mannitol group;Group D:high-dose Mannitol group;Group E:Nimotop group;Group F:Mannitol combined with Nimotop group). Both nitrate reductase and TUNEL methods were respectively used to measure NO content and apoptotic neurons in the brains.Results NO content and apoptotic neurons were higher in group B than those in group A (P<0.01). They were also higher than in group C,D,E and F (all P<0.05). There was no significant difference between group C and D (P>0.05). NO content and apoptotic neurons were lower in group F than in group C,D and E (all P<0.05).Conclusions Low or high dose of Mannitol,Nimotop and combined use both of them may provide neuroprotective effects on ischemic reperfusion injury by decreasing content of NO and inhibiting neural apoptosis in rats. Combined use both of Mannitol and Nimotop has more neuroprotective effects.