血细胞分析仪自动计数外周血有核红细胞的应用研究

The applied research of automatic nucleated red blood cell count in peripheral blood by blood cell analyzer

目的评价血细胞分析仪自动计数外周血有核红细胞(NRBC)的方法学特点,并探讨其临床应用价值。方法选择115例血液病及非血液病患者,用SysmexXE2100全自动血细胞分析仪及显微镜计数法计数其外周血中NRBC数量。结果SysmexXE2100与显微镜计数法计数外周血中NRBC数量有极好的相关性(r>0.97),并且2种方法的计数结果差异无显著意义(P=0.2018)。高值、中值和低值NRBC标本绝对计数的批内CV平均<6.5%。在(0～15)×109/L范围内计数NRBC有极好的线性(r=0.9998)。SysmexXE2100自动计数外周血中NRBC的灵敏度和阴性预测值均为100%。外周血中NRBC增高时,WBC显著高于去除NRBC的计数结果(P=0.0168)。结论SysmexXE2100血细胞分析仪可灵敏、准确、精密地自动计数外周血中NRBC,并且可在NRBC增高的标本中准确计数白细胞数量。除白血病、贫血等血液病患者外,非血液病(如肿瘤化疗)等患者外周血中也可出现NRBC。血细胞分析仪自动计数外周血中NRBC,有助于一些血液病及非血液病患者的筛查与诊断。

Objective To evaluate the characteristics of an automatic nucleated red blood cell (NRBC) count by blood cell analyzer and research its clinical applications.Method The peripheral blood NRBC of 115 patients suffering from hematological diseases or other diseases were counted by both Sysmex XE-21000 automatic blood cell analyzer and manual method with microscope.Results There was a very good correlation between the manual and automatic method (r>0.97) . No significant difference was observed between the counting results of two methods(P=0.201 8). The average intra lot CV of absolute NRBC count of high, medium and low value was below 6.5%. The NRBC counts was rather linear(r=0.999 8) in the range of 0~15 ×10~9/L. Both the sensitivity and negative prediction value were 100%. When the peripheral NRBC increases, the counting of WBC was significantly higher than the value obtained when the NRBC was depleted (P=0.016 8).Conclusions The NRBC counting by the XE-2100 was sensitive, accurate and reliable. The XE-2000 can measure the WBC accurately in sample with increased NRBC. Besides patients with hematological diseases, such as leukemia, anemia etc, the NRBC could also be observed in other patients with non hematological diseases, e.g. cancer chemotherapy. The automatic evaluation of peripheral NRBC maybe helpful to the screening and diagnosis of some hematological and non hematological diseases.