红千层的离体培养及快速繁殖

Tissue Culture and High Frequency Propagation of Callistemon rigidus R.Br

研究了红千层离体快繁的若干影响因素。结果表明:叶片在MS+6BA1.5mg·L-1+NAA0.5mg·L-1中的愈伤组织诱导率43%,并可直接从愈伤组织表面分化出不定芽,分化率为69.2%;腋芽启动培养基为MS+6BA1～1.5mg·L-1+NAA0.5mg·L-1,萌动率为77%;继代和增殖培养基为MS+6BA1mg·L-1+NAA0.25mg·L-1,平均增殖系数为16.7;生根壮苗培养基为1/2MS+NAA0.25mg·L-1,生根率96.1%;试管苗生根培养30～35d移栽,成活率最高可达90%以上。

Effects of several factors such as medium, cutting materials and plant growth regulators on invitro culture and rapid propagation of callistemon rigidus R.Br were investigated. Using the leaf and axillary bud as explant, a method for high frequency propagation of callistemon rigidus R.Br was established. Results showed: (1)MS medium supplemented with 6-BA 1.5 mg·L^(-1)and NAA 0.5 mg·L^(-1) was aptimized for callus induction of leaf. The crowd of bud on callus was differented with the ratio of 69.2%. On MS medium supplemented with 6-BA 1-1.5 mg·L^(-1) and NAA 0.5 mg·L^(-1), the crowd of bud was started with the ratio of 77%.(2) The explants diffedrentiated a large number of young buds on MS medium containing 6-BA 1 mg·L^(-1) and NAA 0.25 mg·L^(-1).On this medium shoot multiplication occurred at a 16.7-fold rate. (3) The shoots were rooted on the 1/2 strength MS medium supplemented with NAA 0.25 mg·L^(-1) and the rate of rooting was 96.1%.The shoots could be directly rooted in transplantation medium with up to 90% survival.