促肝细胞生长素与胰岛素合用对糖尿病大鼠胰岛分泌功能的影响

Effect of the combination of promoting hepatocyte growth factor and insulin to pancreatic islet secretry function of diabetic mice

目的 研究促肝细胞生长素(PHGF)与胰岛素(INS)合用对实验性糖尿病大鼠胰岛分泌功能的影响。方法 链脲霉素性大鼠糖尿病模型75只,分成5组,每组15只:INS(2U·kg-1,im) ;尼克酰胺(1g·kg-1,ig) ;PHGF(2 0mg·kg-1,iv) ;INS(2U·kg-1,im)合并PHGF(2 0mg·kg-1,iv) ;NS(同容量每只1mL ,iv)。3mo后放免法测定血INS和C肽。免疫组化染色法制作病理切片,胰岛β细胞记数并观察切片。结果 与NS组比较,PHGF与INS合用组能显著提高外周血INS和C肽浓度(P <0 . 0 1) ,胰岛β细胞数目显著增多,镜检有大小不等的β细胞。结论 PHGF与INS合用能够明显改善实验性糖尿病大鼠的胰岛β细胞功能,并有胰岛β细胞再生作用。

AIM To study the effect of the combination of promoting hepatocyte growth factor and insulin on pancreatic islet secretry function of diabetic mice.METHODS Seventy-five diabetic mice induced by streptozotocin were divided into 5 groups,fifteen diabetic mice each group.Those groups were separately treated by insulin(2 U·kg -1,im),nicotinamide(1 g·kg -1,ig),promoting hepatocyte growth factor(20 mg·kg -1,iv),promoting hepatocyte growth factor(20 mg·kg -1,iv) combined with insulin(2 U·kg -1,im),NS(same volume,1 mL,iv).Three months later,blood insulin and C-peptide were analysed by radioimmunoassay.Pathological photos were made by immunohistochemical staining and were observed.β cell numbers in them were counted.RESULTS Compared with NS, the combination of promoting hepatocyte growth factor and insulin could significantly improve blood insulin and C-peptide concentrations(P<0.01).Islet β cells were significantly increased. Big or small β cells were observed by microscope.CONCLUSION The combination of promoting hepatocyte growth factor and insulin could significantly improve pancreatic secretry function and have the effect of regenerating β cell.