小鼠单纯疱疹病毒Ⅰ型心肌炎与心肌细胞凋亡和c-Myc蛋白表达的关系

The Relation among Apoptosis of Myocardial Cell and Expression of c-Myc and Herpes Simplex Virus I Type Myocarditis in Mouse

为了探讨小鼠单纯疱疹病毒Ⅰ型心肌炎与心肌细胞凋亡和cMyc蛋白的表达的关系,给BALB/C小鼠腹腔接种单纯疱疹病毒Ⅰ型以诱发其急性病毒性心肌炎(VMC),感染病毒3～35天后,VMC检出率为91.67%。应用电镜、原位末端标记法(TUNEL)及免疫组化技术检测发现,感染鼠心肌中有细胞凋亡和cMyc蛋白的表达,二者阳性率分别为78.33%和81.67%,凋亡细胞阳性指数(AI)范围在34.14±11.56～26.41±10.2之间。细胞凋亡和cMyc蛋白可能参与VMC的发生与发展。

In order to explore the relation among apoptosis of myocardial cell and expression of cMyc and VMC, in this experiment,Viral myocarditis (VMC) in BALB/C mouse was obtained by herpes simplex virus I type that was inoculated into abdominal cavity. The rate of VMC was 91.67% after 3～35 days of virus infection. The apoptosis (78.33%) and expression of cMyc (81.67%) were found in the myocardium of infected mouse by TDT/d UTP Nick End Labeling (TUNEL) and immunohistochemical method and electronmicroscope. AI of myocardial cell is 34.14±11.56～26.41±10.2. Apoptosis and expression of cMyc may participate in occurrence and development of VMC.