长程雾化吸入硝酸甘油对大鼠低氧性肺动脉高压的影响

The effect of long-term nebulized inhalation of nitroglycerin on rats with hypoxic pulmonary hypertension

目的探讨长程雾化吸入硝酸甘油对大鼠低氧性肺动脉高压的阻抑效应及其机制。方法30只Wister大鼠,随机分为:正常对照(Control,C)组、单纯低氧(H)组、低氧+长程雾化吸入硝酸甘油(Neb-NTG,NH)组,观察肺动脉平均压(mPAP)、颈动脉平均压(mCAP)、腺泡内肺动脉3型血管比例、血浆cGMP和ET-1含量、高铁血红蛋白浓度、右心室比重和肺系数。结果NH组的肺动脉平均压、腺泡内肺动脉3型血管比例、血浆cGMP和ET-1含量和右心室比重与H组比较有显著性差异(P<0.001);H组肺动脉平均压、腺泡内肺动脉3型血管比例、血浆cGMP和ET-1含量和右心室比重与C组相比,有显著性差异(P<0.001);但H组、NH组和C组的高铁血红蛋白浓度、颈动脉平均压和肺系数相比较无显著性差异(P>0.05)。结论(1)慢性低氧后肺动脉平均压有明显的升高,但长程雾化吸入硝酸甘油可防止或减弱其升高;(2)长程雾化吸入硝酸甘油可防止或减弱长期低氧所致的肺血管重塑和右心室比重增加;(3)长程雾化吸入硝酸甘油对颈动脉平均压、高铁血红蛋白浓度和肺水含量无明显影响。

Objective To explore the effect of inhibition and mechanism of long-term nebulized inhalation of nitroglycerin on rats with hypoxic pulmonary hypertension. Methods 30 Wister rats were randomly divided into 3 groups: Contrast (Control) group , simple group of hypoxia (H) , group hypoxia +Neb-NTG (NH) normally. Observe mPAP, mCAP , gland lung artery three type blood vessel proportion , plasma cGMP and ET-1 , high iron hemoglobin density , right ventricle proportion and lung coefficient. Results The group NH was significance difference , compared with group H in mPAP and the type three proportion of blood vessel of lung artery, plasma cGMP and ET-1 content in the gland (P<0.001); Group H, the mPAP , type three proportion of blood vessel of lung artery , plasma cGMP and ET-1 content and right ventricle proportion compare with group Control in the gland, was significance difference(P<0.001). But among H group , NH group and control group were not significance difference (P>0.05). Conclusion (1) MPAP increases obviously because of chronic hypoxia,but long-term Neb-NTG contributes to preventing or decreasing MPAP;(2)Long-term Neb-NTG can prevent or subside lung blood vessel remodeling and right ventricle proportion increase derived from long- term hypoxia; (3) Long-term Neb-NTG has no obvious effect on MCAP,the concentration of methemoglobin, pneumonic coefficient.