除臭酵母的筛选及其除臭机理

The Screening of the Deodorization Yeast and the Deodorization Mechanism

以氨气的释放量为主要指标,从初筛获得的具有除臭效果的菌中复筛出一株酵母菌(OCY-1),其在实验室条件下可减少氨气释放量达30%以上,对鸡粪具有明显的除臭效果。OCY-1的发酵最佳培养基为:1.5%葡萄糖+0.5%蔗糖,0.25%蛋白胨+0.25%(NH4)2SO4,0.1%NaCl,0.1%KH2PO4,0.05%CaCl2.2H2O,0.1%MgSO4.7H2O;最佳生长条件为:温度35℃,pH6.0,摇瓶装液量50mL。通过对氮素消除机理分析,发现氨气释放量的减少与鸡粪中NH4+-N剩余量相关,与NO3--N的量不相关。

A special strain (OCY-1) was screened from many types of yeast.The experiment revealed that OCY-1 can decrease NH_(3) emission 30% in the chicken manure.The optimal concentration of carbon resource,pH 6.0,temperature 35℃, 0.1%KH_(2)PO_4, 0.1%MgSO_(4)·7H_(2)O, 0.05% CaCl_(2)·2H_(2)O and 0.1% NaCl of OCY-1 was glucose .Nitrogen resources can be used widely,suggesting to use (NH_(4))_(2)SO_(4).The degrading mechanism of the NH_3 was also studied.It was discovered that there is strong relation between the emission of ammonia and the remains of NH^+_4-N in chicken manure,but no relation to the remains of NO^-_3-N in chicken manure.