环氧化酶-2在胃癌组织中的表达及其与血管生成的关系

Relationship between cyclooxygenase-2 expression and angiogenesis in patients with gastric cancer

目的探讨环氧化酶-2(COX-2)在胃癌组织中的表达及其与肿瘤血管生成的关系。方法应用免疫组织化学(免疫组化)法检测96例胃癌手术切除标本中COX-2的表达,采用抗CD34抗体标记微血管内皮细胞,计算微血管密度(MVD)。分析COX-2表达与MVD和胃癌主要临床病理特征的相关性。结果胃癌组织COX-2阳性表达率和MVD分别为80.2%和32.5±8.3,显著高于正常胃黏膜的13.3%和13.1±2.4;P<0.01。临床TNM分期中Ⅲ、Ⅳ期胃癌标本COX-2表达率与MVD为91.4%和34.9±8.7,显著高于Ⅰ和Ⅱ期胃癌标本(P<0.01)。伴有淋巴结转移的胃癌组织中COX-2表达率和MVD分别为87.9%和35.0±8.5,与无淋巴结转移病例相比,差异有统计学意义(P<0.05)。Spearman等级相关分析表明,COX-2表达与MVD呈显著正相关(γ=0.311,P<0.01)。结论COX-2表达在胃癌的肿瘤血管生成中起重要作用,COX-2及其诱导的血管生成与胃癌的浸润和转移密切相关。

Objective To investigate COX- 2 expression in patients with gastric cancer and its relationship with angiogenesis and clinicopathologic features of gastric cancer. Methods COX- 2 expression and CD34- stained microvessel density (MVD) were detected by immunohistochemical methods in specimens from 96 patients with gastric cancer. The correlations among COX- 2 expression, MVD and clinicopathologic features were analyzed. Results The COX- 2 positive rate and MVD in gastric cancer were significantly higher than those in the normal gastric mucosa (80.2% vs. 13.3% ;32.5± 8.3 vs. 13.1± 2.4, all P< 0.01). The COX- 2 positive rate and MVD in the patients with stage Ⅲ and Ⅳ were significantly higher (91.4% and 34.9± 8.7 respectively,P< 0.01),than that in the patients with stage Ⅰ and Ⅱ . The COX- 2 positive rate and MVD in the cases with lymph node metastasis were 87.9% and (35.0± 8.5) respectively, higher than those in the cases without lymph node metastasis (P< 0.05). The Spearman rank correlation test showed a significant correlation between COX- 2 expression and tumor MVD (r=0.311, P< 0.01). Conclusions COX- 2 plays an important role in gastric cancer angiogenesis. COX- 2 and angiogenesis induced by COX- 2 contribute to tumor invasion and lymph node metastasis.