摘要
目的应用液相色谱/电喷雾离子化质谱法同时分析几种大环内酯类抗生素。方法用HypersilODS2(250×4.6mm,5μm)色谱柱;以甲醇∶水∶三乙胺(68∶32∶0.1,V/V)为流动相用1mL·min-1的流速洗脱;DAD检测器检测波长230nm;柱温40℃;液相出口分流20%进入离子阱质谱,以电喷雾电离源正离子模式(MS+)进行质谱数据采集。结果得到样品液相色谱图和质谱图,并对谱图进行分析和归属。结论本方法方便、快速、灵敏、可靠,对临床药学、食品安全和生态环境等的检测方法的研究具有一定的指导意义。
OBJECTIVE Sample containing several macrolide antibiotics samples was analyzed by using liquid chromatorgraphy-electrospray ionization mass spectrometry.METHODS column:Hypersil ODS_2(250×4.6mm,5μm);mobile phase:CH_3OH-H_2O-triethylmine(65∶35∶0.1,V/V);flow rate:1mL·min -1 ;wavelength:227nm;temperature:40℃;20% of the exit from liquid chromatorgraphy was pumped into the ion trap inside,the mass spectrometry and the mass spectral of components of the sample was analyzed.RESULTS Leucomycin A_6,midecamycin A_1,erythromycin A,josamycin,clarithromycin,roxithromycin,acetylspiramycins were detected in the sample according to LC-MS result.CONCLUSION LC-MS method is a sensitive,rapid and credible method for the analysis of macrolide antibiotics.
出处
《海峡药学》
2005年第3期35-40,共6页
Strait Pharmaceutical Journal
